hemagglutination, hemolysis Flashcards
refers to the clumping of particles caused by the union of specific antibody molecules with particulate antigens expressed on a cell surface
agglutination
if the source of the antigens are red cells and these cells are agglutinated by their corresponding antibodies, this is called
hemagglutination
principle: when particulate __ expressed on the __ combine with the specific __ that are present in __ samples, clumping of cells will occur
antigens, RCS
antibodies, serum
4+
3+
2+
1+
w+ (weak)
mixed field rxn
negative`
if clumping is not too evident, perform a __
microscopic observation
red cells that pile up like stack of coins; observed when plasma is used instead of serum
rouleaux formation
if the cells disperse when the NSS is added, it’s identified as
true rouleaux
t/f: if discoloration/hemolysis is observed, it should be noted
t
reciprocal of the maximum dilution that gives visible agglutination
titer
well with the lowest concentration of the antibodies in which there is agglutination
endpoint
4+
3+
2+
1+
+/-
rupturing or destruction of RBCs resulting from the interaction of antibody-coated RBCs with several serum factors of the C’ system
hemolysis
t/f: inactivated serum is used in hemolysis determination
t`
freshly collected sample
sample x
