determination of c3

Question 1

the __ is a collection of circulating and membrane-associated proteins constitutively present in the blood and other body fluids in a biologically inactive form

Answer 1

complement system

Question 2

complement analysis has been associated with the accurate measurement of __, __, and __ substances that constitute all the three pathways of the complement system

Answer 2

complement proteins, enzymes, regulatory substances

Question 3

t/f: screening tests for complement activity intended for the diagnosis of the complement deficiencies are not available in routine diagnostic laboratories

Answer 3

f (are also available)

Question 4

two types of assayes performed in determining and analyzing complement components

Answer 4

antigenic and functional assay

Answer 5

antigenic assay

Answer 6

antigenic assay

Answer 7

functional assay

Answer 8

functional assay

Answer 9

functional assay

Question 10

__ is precipitation in a gel medium that mainly evolved from the works of __ and __

Answer 10

immunodiffusion, john fahey, eugene mckelvey

Question 11

t/f: immunodiffusion is a diagnostic test intended for the identification of antigens or for the qualitative study of antibodies

Answer 11

f (quantitative)

Question 12

c3 is also known __

Answer 12

b1c globulin

Question 13

c3 constitutes __ of the total proteins in the complement system

Answer 13

70%

Question 14

measuring c3 has become a routine practice and is tested alongside __ and __

Answer 14

c1q, c4

Question 15

three proteins useful in monitoring inflammatory conditions, such as nephritis and certain immune diseases, such as systemic lupus erythematosus

Answer 15

c3, c1q, c4

Question 16

what component is intoduced to a gel medium

Answer 16

c3

Question 17

when human __ present in a serum sample is introduced to a gel medium, it diffuses from the circular well through the homogenous gel containing the monospecific __ antibody

Answer 17

c3, anti-c3

Question 18

the size of the precipitin ring continues to grow until __ is reached

Answer 18

equilibrium point

Question 19

c3 values obtained are expressed in __

Answer 19

mg/dL

Question 20

expected range of value for c3

Answer 20

37-404mg/dL

Question 21

when thr size or diameter of the zone or precipitin ring of the test sample exceed that of the highest calibrator or standara, the specimen should be __

Answer 21

diluted

Question 22

if the diameter of the zone or precipitin ring of the test sample is smaller than that of the lowest standard, the specimen should be reported as __

Answer 22

less than the concentration of the reference

Question 23

serologic reaction in which particulate antigens suspended in fluid form clumps or aggregate in response to the presence of specific antibodies

Answer 23

agglutination

Answer 24

direct agglutination

Question 25

[type of agglutination]: intended to detect antibodies in a person's serum with the use of a known antigen

Answer 25

indirect / passive agglutination

Question 26

[type of indirect agglutination]: utilizes a known antigen that coats onto latex or other inert particles to identify antibodies on a patient's serum

Answer 26

classic passive agglutination

Question 27

[type of indirect agglutination]: a type of agglutination wherein the antibody, rather than the antigen, is covalently linked to the carier particle and mixed with a patient's serum in order to detect the presence of the unknown antigen

Answer 27

reverese passive agglutination

Question 28

[type of agglutination]: highly sensitive for identifying small amounts of antigens in a patient's sample; used for epidemiological studies of some viral diseases and home-based pregnancy tests

Answer 28

agglutination inhibition

Question 29

t/f: agglutination inhibition is used as a premarital test to determine the immune statys of females against rubella infection

Answer 29

t (in some countries)

Question 30

a classic method of detecting antigen-specific antibodies

Answer 30

complement fixation

Question 31

t/f: complement fixation is composed of three components

Answer 31

f (two components)

Question 32

[first or second component of C' fixation]: antigen-antibody reaction

Answer 32

first

Question 33

[first or second component of C' fixation]: known as the indicator system

Answer 33

second

Question 34

[first or second component of C' fixation]: suspension of sheep RBC and anti-sheep antibody is added to determine whether the complement was fixed or taken up in the first test system

Answer 34

second

Question 35

positive result of complement fixation

Answer 35

agglutination

Question 36

type of serologic reaction wherein specific antibodies are able to block or nullify the chemical activity or biological effects of the antigen

Answer 36

neutralization

Question 37

in a serologic assay, the neutralization or detection of the presence of neutralizing antibodies can be demonstrated by adding __ as indicator cells to the antigen-antibody mixture

Answer 37

suspensions of RBCs

Question 38

t/f: neutralization and complement fixation have the same results

Answer 38

t