Sequencing Quick Info Flashcards
1
Q
3 Proteases used for larger sequencing
A
Trypsin
Chymotrypsin
Carboxypeptidase
2
Q
Edman degradation
A
removes the N-terminal amino acid
If you have 2 n-terminal amino acids, probably have a disulfide bond
3
Q
Trypsin
A
a protease that cuts after positively charged amino acids
4
Q
Chymotrypsin
A
a protease that cuts after an aromatic group
5
Q
Carboxypeptidase
A
cuts away the C-terminal amino acid
6
Q
Reducing agents for disulfide bonds
A
B-mercaptoethanol (B-ME) and dithiothreitol (DTT)
7
Q
Cyanobromide
A
a reagant (way to break a protein without a protease)
breaks a sequence after a methionine
