Seperation Techniques Flashcards
What are the 8 techniques to be made aware of
NMR Xray Diffraction Mass spectroscopy Thermal analysis Chromatography UV analysis Infrared IR analysis Microscopy
What is separation is affected by
differences in distribution
mobility of molecules
What are the 3 separation techniques to be made aware of
Electrophoresis
Membranous
Chromatography
What is gel electrophoresis
lab technique used to separate DNA or proteins based on size
How does gel electrophoresis work
Produce electrical field with cathode and anode on opposite ends of the box which contains the gel and the buffer.
The samples split forming a DNA ladder we then compare it to see what fragments mean what
What is membrane separation dependent on
size, the small molecules are removed down a concentration gradient hile the larger ones are left behind.
what is membrane separation used for
commonly used to remove impurities and small dye molecules from polymers or particles
What is chromatography
components of a mixture are separated based on differences in the rate at which they are carried through a stationary phase by a gaseous or liquid mobile phase
what is the stationary phase
fixed in place either in a column or on a planar surface
what is the mobile phase
moves or or through the stationary phase carrying with it the analyte mixture
What are the two types of High performance liquid chromatography
Normal phase
Reverse phase
what is normal phase
it is when there is a polar stationary phase and a non polar mobile phase
what is reverse phase
it is when there is a non polar stationary phase and a polar mobile phase
What is elution
process of washing sample components through the stationary phase by continuous flow of the mobile phase
how is retention time denoted
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