Semen

Question 1

What is the largest fraction in the composition of semen fluid?

Answer 1

Seminal fluid (60-70%)

Question 2

Where do the sperm mature and develop flagella?

Answer 2

Epididymis

Question 3

The seminal fluid contains a high concentration of ____ & ____.

Answer 3

Fructose; flavin

Question 4

Approximately 20-30% of the semen volume is ____ fluid produced by the prostate gland.

Answer 4

Acidic

Question 5

The prostate fluid is responsible for both ____ & ____ of the semen following ejaculation.

Answer 5

Coagulation; liquefaction

Question 6

First portion of ejaculate missing: ____ sperm count, ____ pH, specimen will ____

Answer 6

Decreased; increased; liquefy

Question 7

Last portion of ejaculate missing: ____ sperm count, ____ volume, ____ pH, specimen will ____

Answer 7

Increased; decreased; decreased; clot

Question 8

Specimens are collected following a period of sexual abstinence of at least ___ days to not more than ___ days.

Answer 8

2; 7

Question 9

Specimens are discarded as ____ waste.

Answer 9

Biohazardous

Question 10

A fresh specimen is clotted and should liquefy within ____ minutes.

Answer 10

30-60

Question 11

If after 2 hours the specimen has not liquefied, what may be added to induce liquefaction?

Answer 11

Dulbecco’s phosphate-buffered saline or proteolytic enzymes (chymotrypsin or bromelain)

Question 12

pH should be measured within ____ of ejaculation due to the loss of Co2 that occurs.

Answer 12

1 hour

Question 13

Count greater than ____ leukocytes per mL is associated with inflammation or infection of the reproductive organs.

Answer 13

1 million

Question 14

Presence of more than 1 million ____ per mL indicates disruption of spermatogenesis.

Answer 14

Spermatids

Question 15

Motility is evaluated by both ____ & ____.

Answer 15

Speed; direction

Question 16

This instrumentation provides objective interpretation of both sperm velocity and trajectory, as well as sperm concentration and morphology

Answer 16

Computer-assisted semen analysis (CASA)

Question 17

Sperm motility grade: slower speed, some lateral movement

Answer 17

3

Question 18

Sperm motility grade: slow forward progression, noticeable lateral movement

Answer 18

2

Question 19

The normal sperm has an oval-shaped head approximately ___ um long & ___ um wide.

Answer 19

5; 3

Question 20

The flagellar tail of the sperm is approximately ___ um long.

Answer 20

45

Question 21

The acrosomal cap should encompass approximately ____ of the head and cover approximately ____ of the sperm nucleus.

Answer 21

Half; 2/3

Question 22

The midpiece is approximately ___ um long and is the thickest part of the tail.

Answer 22

7

Question 23

____ are the predominant form of leukocyte in semen.

Answer 23

Peroxidase-positive granulocytes

Question 24

Presence of a large proportion of vital but immobile cells may indicate a defective ____.

Answer 24

Flagellum

Question 25

High number of immotile and nonviable cells may indicate ____ pathology.

Answer 25

Epididymal

Question 26

In the immunobead test, head-directed antibodies can interfere with ____, whereas tail–directed antibodies affect ____.

Answer 26

Penetration; movement

Question 27

Motile sperm is present up to ____ after intercourse, while nonmotile sperm is present up to ____.

Answer 27

24 hours; 3 days

Question 28

Heads of the sperm once they die off are still present for up to ____ after intercourse.

Answer 28

7 days

Question 29

A more specific method than ACP is the detection of ____, which is present even in the absence of sperm.

Answer 29

Seminal glycoprotein 30/PSA

Question 30

This is a test for choline using iodine crystals & KI as reagents

Answer 30

Florence test

Question 31

This is a test for spermine with saturated picric acid & TCA as reagents

Answer 31

Barbiero’s test