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Microbiology Lab > Selective and differential experiment quiz > Flashcards

Selective and differential experiment quiz Flashcards

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Biology 101 flashcards
1
Q

what is the definition of selective media?

A

inhibits growth of unwanted microbes (only the selected microbes live)

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2
Q

what is the definition of differential media?

A

allows organisms to grow but the added chemicals make them look different (they change different colors and/or precipitate is visible)

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3
Q

what were the three specific plates that we used in our lab?

A

MSA: Mannitol salt agar
EMB: Eosin methylene blue
MAC: MacConkey agar

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4
Q

where were the colors of the MSA, EMB, and MAC plates?

A

MSA: red
EMB: dark purple
MAC: raspberry

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5
Q

which plate used in lab this semester was not selective or differential?

A

TSA

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6
Q

which organisms used in this exercise are Gram + ?

A

Staphylococcus aureus
Staphylococcus epidermidis

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7
Q

which organisms used in this exercise are Gram - ?

A

Escherichia coli
Enterobacter aerogenes
Proteus vulgaris

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8
Q

How is MSA selective?

A

selects for gram +
selects against gram -

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9
Q

how is EMB selective?

A

selects for gram -
selects against gram +

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10
Q

how is MAC selective?

A

selects for gram -
selects against gram +

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11
Q

How is MSA differential? (fermentation?)

A

differential for mannitol fermentation

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12
Q

how is EMB differential? (fermentation?)

A

differential for lactose fermentation

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13
Q

how is MAC differential? (fermentation?)

A

differential for lactose fermentation

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14
Q

how is MSA differential? (color changes?/type of bacteria)

A

fermenter: growth turns yellow (Staphylococcus aureus)
nonfermenter: growth remains red (Staphylococcus epidermidis)

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15
Q

how is EMB differential? (color changes?/type of bacteria)

A

strong fermenter: growth turns shiny metallic green (Escherichia coli)
weak fermenter: growth turns pink around the edges of colonies (Enterobacter aerogenes)
nonfermenter: growth remains dark purple (Proteus vulgaris)

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16
Q

how is MAC differential? (color changes?/types of bacteria)

A

strong fermenter: entire plate turns bright pink (Escherichia coli)
weak fermenter: growth turns light pink with precipitate and background turns light brown (Enterobacter aerogenes)
nonfermenter: entire plate turns brown (Proteus vulgaris)

Microbiology Lab (11 decks)

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