Bacterial Transformation Quiz Flashcards
What is bacterial transformation?
The uptake of naked DNA molecules by bacterial cells
What is competence?
The ABILITY of bacterium to take up naked DNA molecules
Which bacteria are naturally competent?
Streptococcus pneumoniae
Bacillus subtilis
What is an example of a bacteria that is not naturally competent?
Escherichia coli
What are we transforming cells with?
pGLO plasmid
How will we increase competence for E. coli?
Treating with calcium chloride and heat shock
What bacteria are we transforming in lab?
Escherichia coli
What are the observable traits the plasmid will offer?
(antibiotic) ampicillin-resistant gene
fluorescent protein gene
What gene is the specific “foreign” gene? Where does it come from
GFP gene
A jellyfish (Aequorea victoria)
How many plates will we be inoculating?
3
What is the purpose of the ice bath and water bath?
To subject it to heat shock in order to increase competence
What is the purpose of araC gene?
To enable the GFP gene to be able to activate
