Section Bank BB Flashcards

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1
Q

how to distinguish southern blot sequences

A

a Southern blot uses a restriction digest to differentiate between mutant and wild-type alleles. In order for a Southern blot to be useful, the mutation should either create or eliminate a restriction site, most of which are palindromes and 4 to 6 base pairs long. The mutation shown in this option is the only one that disrupts a palindromic sequence, AAGCTT

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2
Q

the average molecular weight of an amino acid is

A

110 Da

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3
Q

hill coefficient

A

n>1 = cooperativity, n=1 no cooperativity n<1 = negative cooperativity

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4
Q

tertiary stabilization

A

phosphodiester bonds stablize primary, NOT tertiary

salt bridges are the ion interactions with proteins

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5
Q

uncompetitive

A

decrease both Km and Vmax
uncompetitive inhibitors bind their target enzymes only when the substrate is first bound to the enzyme. Since at higher substrate concentrations, the substrate–enzyme complex are more abundant, the uncompetitive inhibitor will work most effectively when the substrate concentration is the highest. Additionally, an increase in the inhibitor concentration results in increased enzyme binding and inhibition.

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6
Q

signal sequence domans

A

signal sequence domains are protein domains required for proteins that are directed toward secretory pathways

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7
Q

what proteins are included in dimerization

A

polar and charged amino acids most likely interact with water molecules in cytosol and would not be involved in protein-protein interactions. In contrast the side chains of hydrophobic amino acids are free and most likely participate in dimerization of STAT3.

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8
Q

weight of AA

A

110 Da

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9
Q

Which methods separate proteins based on their charge?

A

isoelectric, ion exchange

-NOT sds page, affinity

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10
Q

Which enzyme is used both in gluconeogenesis and glycogenolysis?

A

gluco 6 phosphatase

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11
Q

hypertension

A

high BP

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12
Q

cDNA cloning

A

DNapol–> RNA–>post modify–> reverse transcriptase back to cDNA
-ligase connects

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13
Q

starting materials for gluconogenesis

A

oxoloacetate, alpha ketoglutarate, lactate, glycerol

-NOT acetyl coa, phosphogluconate

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14
Q

glycogen breakdown enzyme

A

Glycogen phosphorylase is the enzyme that catalyzes the rate-limiting step in glycogen breakdown (glycogenolysis).

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15
Q

coding strand and tempalte strand

A

coding and mRNA are comp to template

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