Section Bank BB

Question 1

how to distinguish southern blot sequences

Answer 1

a Southern blot uses a restriction digest to differentiate between mutant and wild-type alleles. In order for a Southern blot to be useful, the mutation should either create or eliminate a restriction site, most of which are palindromes and 4 to 6 base pairs long. The mutation shown in this option is the only one that disrupts a palindromic sequence, AAGCTT

Question 2

the average molecular weight of an amino acid is

Answer 2

110 Da

Question 3

hill coefficient

Answer 3

n>1 = cooperativity, n=1 no cooperativity n<1 = negative cooperativity

Question 4

tertiary stabilization

Answer 4

phosphodiester bonds stablize primary, NOT tertiary

salt bridges are the ion interactions with proteins

Question 5

uncompetitive

Answer 5

decrease both Km and Vmax
uncompetitive inhibitors bind their target enzymes only when the substrate is first bound to the enzyme. Since at higher substrate concentrations, the substrate–enzyme complex are more abundant, the uncompetitive inhibitor will work most effectively when the substrate concentration is the highest. Additionally, an increase in the inhibitor concentration results in increased enzyme binding and inhibition.

Question 6

signal sequence domans

Answer 6

signal sequence domains are protein domains required for proteins that are directed toward secretory pathways

Question 7

what proteins are included in dimerization

Answer 7

polar and charged amino acids most likely interact with water molecules in cytosol and would not be involved in protein-protein interactions. In contrast the side chains of hydrophobic amino acids are free and most likely participate in dimerization of STAT3.

Question 8

weight of AA

Answer 8

110 Da

Question 9

Which methods separate proteins based on their charge?

Answer 9

isoelectric, ion exchange

-NOT sds page, affinity

Question 10

Which enzyme is used both in gluconeogenesis and glycogenolysis?

Answer 10

gluco 6 phosphatase

Question 11

hypertension

Answer 11

high BP

Question 12

cDNA cloning

Answer 12

DNapol–> RNA–>post modify–> reverse transcriptase back to cDNA
-ligase connects

Question 13

starting materials for gluconogenesis

Answer 13

oxoloacetate, alpha ketoglutarate, lactate, glycerol

-NOT acetyl coa, phosphogluconate

Question 14

glycogen breakdown enzyme

Answer 14

Glycogen phosphorylase is the enzyme that catalyzes the rate-limiting step in glycogen breakdown (glycogenolysis).

Question 15

coding strand and tempalte strand

Answer 15

coding and mRNA are comp to template