Chem Section Bank

Question 1

mixing 2 solutions

Answer 1

DO NOT FORGET you everage the two M, you have gotten this wrong multiple times

Question 2

transferase

Answer 2

a kinase that catalyzes the transfer phosphate groups from ATP to target proteins

Question 3

catalytic efficiency

Answer 3

Kcat/Km

Question 4

uncompetetive inhibitor

Answer 4

uncompetitive inhibitor results in a lower apparent KM and Vmax of the enzymatically catalyzed reaction.

Question 5

Histidine

Answer 5

Do not count as +1

Question 6

anion exchange

Answer 6

anionic peptides bind to anionic exchange

Question 7

affinity chrom

Answer 7

the use of histine tagging and a nickel column is a form of affinity chromatography.

highly specific interaction between antigen and antibody, enzyme and substrate, receptor and ligand, or protein and nucleic acid.

Question 8

4 bases

Answer 8

C has the most H bond acceptors

G has the highest MW

Question 9

how many mol NADH to break disulfide bond

Answer 9

1 mol/bond

Question 10

Tm

Answer 10

the temperatire that 50% has melted

Question 11

if given M and need to find volume

Answer 11

use M1V1=M2V2

Question 12

if an enzyme has a lower Km

Answer 12

it probabbly has a lower vmax

Question 13

ternary complex

Answer 13

A ternary complex can be a complex formed between two substrate molecules and an enzyme.

Question 14

viscosity realtionship to diameter

Answer 14

V increases with D because more molecuels interact with the increased surface area of the vessel