Secretion Systems

Question 1

T1SS has 3 parts:

Answer 1

ATP-binding cassette

Outer membrane factor (OMF/OMP)

Membrane fusion protein (MFP)

Question 2

T1SS

Example secreted proteins

Answer 2

Salmonella enterica- secretes SiiE and BapA

Question 3

MFP links _________ to ___________ and is found in type ________SS

Answer 3

• ABC
• OMP
• Type 1SS

Question 4

ABC in T1SS _______________+________ protein

Answer 4

Unfolds + translocates the protein

Question 5

Once protein bound the MFP

Answer 5

Associates tightly with OMP

Question 6

T2SS

Examples of secreted:

Answer 6

Cholera Toxin

Enterotoxin A

Question 7

T2SS

Or _________

Answer 7

Sec dependant pathway

Question 8

T2SS found in _______ (bacteria type)

Answer 8

Gram neg proteobacteria

Question 9

T2SS in pseudomonas have _________

And ______in v.cholera

Answer 9

• 15 different subtypes

- 20 different subtypes

Question 10

T2SS
Required for virulence in 
\_\_\_\_\_
\_\_\_\_\_
\_\_\_\_\_

Answer 10

V. Cholera
Enterotoxogenic E.coli
Pseudomonas

Question 11

T2SS
Span _______ membranes
But…

Answer 11

Both

But… CM only spanned to anchor

Question 12

T3SS

Or

Answer 12

Injectosome

Question 13

T3SS

Found in

Answer 13

Pathogenic and non pathogenic Gram (-)

Question 14

T2SS inhibited by

Answer 14

Guarding mine

Question 15

T3SS is _______ dependant

Answer 15

ATP-

Question 16

T3SS

Found in

Answer 16

Yersina

Question 17

T4SS

Transports

Answer 17

Nucleotides and proteins

Question 18

T4SS

__________competence

Answer 18

Associated with natural

Ie. transformation

Question 19

T4SS Gram ______ cells

Answer 19

(-) and (+)

Question 20

T4SS

Found in

Answer 20

E.coli
Helictobacter
Pseudomonas aeruginosa
Bordetella Pertussis

Question 21

T5SS

Used by ______ to secrete ______

Answer 21

Yersina

YadA

Question 22

T5SS

Found in

Answer 22

E.coli

Yersina

Question 23

T5SS range of proteins dependant on

Answer 23

Plasma encoded virulence factors

Question 24

T6SS

Gram _____

Answer 24

(-)

Question 25

T655 delivers

Answer 25

Effector proteins

Question 26

T6SS
Most similar to ________
Both like ______

Answer 26

T3SS

Phage tail

Question 27

Found in many proteobacteria

Answer 27

T6SS

Question 28

V.cholera and P.aerugenosa have

Answer 28

T6SS

Question 29

T7SS specialized for

Answer 29

Mycobacterium

Question 30

T7SS

________ +__________ facilitate substrate secretion they are found in the _________

Answer 30

EspG and EccA

Cytoplasm

Question 31

T7SS substraits

Answer 31

EsxA

EsxB

Question 32

Substrate processing in periplasm

Answer 32

T7SS

Question 33

Yops use _______and are found in ______they are a type of _________

Answer 33

T3SS
Yersina
Effector protein

Question 34

YadA uses a

Answer 34

T5SS