RP2 - Root Flashcards
1
Q
Hazards
A
Scalpel
Cut down away from yourself on a hard cutting tile
Hydrochloric acid
Wear goggles and wash hands if any contact
2
Q
Why do we use hydrochloric
A
Softens the root
3
Q
Why do we use root tip
A
Where mitosis occurs as it is the growing part of cell
4
Q
Why do we use a mounted needle
A
To avoid air bubbles when placing glass slide
5
Q
Why do we use a stain
A
Stain the chromosomes so they can be easily seen
6
Q
Method
A
- Leave plants to grow in water beaker in the dark
- After 3 days will be root growth
- Place root in HCL and water bath at 60c to soften
- Leave for ten mins
- Cut root 5mm from end
- Rinse root with distilled water
- Add stain so chromosomes can be seen
- Mounted needle uses to placed cover slip to avoid air bubbles
7
Q
Mitotic index
A
Number of cells in mitosis
Total number of cells
8
Q
Why do you press down firmly on cover slip
A
Thin layer of cells so light can pass through
9
Q
When counting cells to calc mitotic index what should you do to ensure your count is accurate
A
Examine large number of fields of view
Representative
