RP 12 TLC Flashcards
1
Q
What are the uses of thin layer or paper chromatography
A
Separate a mixture for analysis
Identification by comparing Rf values
2
Q
How to carry out TLC
A
Draw a pencil line on TLC plate. Spot samples along line using capillary tube
Add TLC plate to a jar with solvent that sits below origin. Add lid
As solvent rises up TLC, components are brought up with it
When solvent reaches nearly top of paper take it out and draw a pencil line then allow to dry
3
Q
What happens if sample to conc
A
Spots overlap
4
Q
What happens if you use less solvent and have a high bassline
A
Large spots
5
Q
How do you view spots on TLC
A
UV light
Sprayed with ninhydrin
