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routine hematology procedures Flashcards

1
Q

routine hema procedure that is not part of CBC

a. hbg
b. plt count
c. rbc count

A

plt count

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2
Q

reagents of cyanmethemoglobin that decrease amount of turbidity resulting from abnormal proteins and improve rbc lysis

a. KCN
b. potassium ferricyanide
c. KH2PO4
d. non ionic detergent

A

nonionic detergent

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3
Q

reagents of cyanmethemoglobin that replaced the Sodium Bicarbonate

a. KCN
b. potassium ferricyanide
c. KH2PO4
d. non ionic detergent

A

KH2PO4

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4
Q

reagents of cyanmethemoglobin that shortens the reaction time instead of 15 mins to 3mins

a. KCN
b. potassium ferricyanide
c. KH2PO4
d. non ionic detergent

A

KH2PO4

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5
Q

methemoglobin + potassium cyanide =

a. cyanmethemoglobin
b. sulfhemoglobin
b. methemoglobin

A

cyanmethemoglobin

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6
Q

what is the remedy for Lipemic sample

a. centrifuge the mixture and use the supernatant
b. add 0.01ml of patient plasma to 5.0ml of HiCn reagent and use this as a blank
c. dilute mixture 1:1 with H2O then multiply the results to 2

A

Lipemic sample

b. add 0.01ml of patient plasma to 5.0ml of HiCn reagent and use this as a blank

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7
Q

what is the remedy for HgB S and HgB C

a. centrifuge the mixture and use the supernatant
b. add 0.01ml of patient plasma to 5.0ml of HiCn reagent and use this as a blank
c. dilute mixture 1:1 with H2O then multiply the results to 2

A

hgb s ang hgb c
c. dilute mixture 1:1 with H2O then multiply the results to 2

high wbc and plt count ———–>centrifuge the mixture and use the supernatant

lipemic sample ———> add 0.01ml of patient plasma to 5.0ml of HiCn reagent and use this as a blank

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8
Q

how many oxygen does 1g of hbg have?

a. 1.34mL
b. 1.45 mL
c. 1.24mL

A

1.34mL

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9
Q

how many Fe++ does 100g of hgb have?

a. 0.345g
b. 1.34 ml
c. 3.37 mg

A

0.345g of Fe++ = 100g of Hgb

1g hgb = 3.47mg iron

1g hgb = 1.34ml of oxygen

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10
Q

method o fcyanmethemoglobin that uses in bloodbank to screen the blood donors

a. blood oxygen capacity
b. colorimetric method
c. copper sulfate method

A

copper sulfate method

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11
Q

what is the specific gravity of the copper sulfate solution

a. 1.053
b. 1.009
c. 1.043

A

1.053

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12
Q

van slyke method

a. blood oxygen capacity
b. blood iron content
c. copper sulfate method

A

blood oxygen capacity

gasometric/ vam slyke method

it uses VAN SLYKE - NEIL APPARATUS or the NATELSON MICRO GASOMETER

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13
Q

measures the functional hemoglobin only

a. blood oxygen capacity
b. blood iron content
c. copper sulfate method

A

blood oxygen content

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14
Q

what is the reagent used in ACID HEMATIN

a, 0.1 N HCL
b. 0.1 N NaOH

A

0.1 N HCL

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15
Q

what is the reagent used in alkali hematin

a. 0.1 N HCL
b. 0.1 N NaOH

A

0.1 N NaOH

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16
Q

sahli’s method

a. copper sulfate method
b. acid hematin
c. alkali hematin

A

acid hematin

17
Q

Gravimetric method

a. copper sulfate method
b. blood oxygen content
c. acid hematin

A

copper sulfate method

18
Q

abnormla hgb pigments mostly acquired due to exposure to certain drugs and chemicals

a. carboxyhgb
b. methemoglobin
c. sulfhahgb

A

methemoglobin

19
Q

it is abnormal pigments of hemoglobin that is thought to be formed by the action of sulfonamides and aromatic amines

a. carboxyhgb
b, methemoglbn
c. sulfhahgb

20
Q

test to detect the Carboxyhgb

a. copper sulfate method
b. sahli’s mthod
c. katayama’s test

A

katayama’s test

  • it uses AMMONIUM SULFIDE as a reagent and will detect as little as 10% of carboxyhgb conc
    (+) carboxyhgb = ROSE RED
21
Q

DIAPHORASE DEFICIENY

a. inherited methemoglbn
b. acquired methemoglbn

A

inherited methemoglobin

most commonly attributed to NADPH METHEMOGLBN REDUCTASE DEFICIENCY, also called DIAPHORASE DEFICIENCY

22
Q

EXPOSURE TO CERTAIN DRUGS AND CHEMICALS

A. Inherited methemoglbn
b. acquired methemoglbn

A

ACQUIRED METHEMOGLBN

23
Q

ANTICOAGULANT USED IN METHEMOGLOBIN

a. sps
b. heparin
c. edta

A

edta or heparin

24
Q

it is abnormal pigment of hgb that is associated with severe constipation and in bacteremia with Clostridium welchii

a. carboxyhgb
b. methemoglbn
c. sulfhahgb

25
Q

What is the size of the internal bore of the wintrobe tube?

a. 3mm
b, 1.2 mm

A

3mm

long= 115mm

26
Q

how long does microhematocrit is?

a, 115mm

b. 75mm
c. 90 mm

27
Q

how CLAY SEAL should it be?

a. 2-4 mm
b, 1-6 mm
c. 4-6 mm

A

4-6 mm long

28
Q

what is the dilution for RBC UNOPETTE

A. 1:20
B. 1:200
C 1:2000

A

1:200

WBC DILUTION- 1:20

29
Q

what are the RBC DILUTING FLUID

G
E
N

T
S
B

H
D

A

RBC DILUTING FLUID (GEN TSB Hayem Dacie)

G=GOWER
E= EAGLE
N=NSS

T=TOISON
S= STRONG
B= BETHEL

HAYEM
DACIE

30
Q

what are the diluting fluid of wbc

A
  1. 2% acetic acid
  2. 1% HCL
  3. Turk’s diluting fluid

Hematology (10 decks)

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