RNAi

Question 1

A target RNA-morpholino hybrid does not promote mRNA degradation because:
-it is relatively unstable
-it could promote general mRNA destruction
-it is a necessary safety precaution in their design
-it is not a substrate for RNaseH

Answer 1

It is not a sibstrate for RNaseH

Question 2

miRNA are found in plants but not in animals- T/F

Answer 2

True

Question 3

MicroRNAs are processed by the enzymes x and y. One of strand of the microRNA is loaded into silencing complex named —-. The microRNAs target mRNA for -
or -
Select one:
* Drosha, Dicer, RISC, mRNA degradation, translational repression
* Drosha, Dicer, RISC, alternative splicing, RNA editing
O RISC, Dicer, Drosha, mRNA degradation, translational repression
* RISC, Dicer, Drosha, alternative splicing, RNA editing

Answer 3

Drosha, Dicer, RISC, mRNA degradation, translational repression

Question 4

RNA interference is thought to have evolved to

Select one:
* promote transposition
* downregulate cancer-causing genes
* prevent spurious recombination
-defend against viruses

Answer 4

defend against viruses

Question 5

Which component of the RNAi pathway confers inheritance of siRNA-induced silencing?
Select one:
* Drosha
* RdRP (RNA-directed RNA polymerase)
* Dicer
* RISC (RNA induced silencing complex)

Answer 5

RdRP (RNA-directed RNA polymerase)

Question 6

siRNA is an abbreviated version of:
Select one:
* small interfering RNA
* small inhibitory RNA
* small inverting RNA
* strongly interfering RNA

Answer 6

-small interfering RNA

Question 7

The targets of a microRNA are usually tissue-specific genes
Select one:
* True
* False

Answer 7

True

Question 8

Antisense oligonucleotides suppress gene expression relatively poorly, in part because of:
of
ion
Select one:
* Both of the above
* inefficient delivery to the target mRNA
* inefficient delivery to cells
* Insufficient target sequences

Answer 8

Both of the above

Question 9

Dicer is the component of the RNAi pathway that confers inheritance of siRNA-induced silencing?
Select one:
-True
* False

Answer 9

True

Question 10

The target mRNA for a particular miNA is cleaved by
-lag question
Select one:
* Drosha
* RISC
* Dicer
* RNA-directed RNA polymerase

Answer 10

RISC

Question 11

Morpholino oligonucleotides have proved useful for
Select one:
* reverse transcriptase-PCR
*RNA interference (RNAi)
* RNase protection assays
* antisense-mediated inhibition of gene expression

Answer 11

RNA interference

Question 12

The targets of a microRNA are usually
Select one:
-viral mRNAs
-“housekeeping” genes
- tissue-specific genes
-bacterial dsRNAs

Answer 12

-tissue specific genes

Question 13

RNA interference is the process of
Select one:
* gene silencing at the level of RNA splicing.
*gene silencing at the transcriptional level.
* gene silencing at the post-transcriptional level.
* gene silencing at the post-translational level

Answer 13

gene silencing at the post-transcriptional level

Question 14

RNA interference is the process of gene silencing at the level of RNA splicing.
* Select one:
* True
* False

Answer 14

False