CRISPR

Question 1

CAS genes makes helicases and endonuclease -T/F

Answer 1

True

Question 2

The interspace between the short palindromic repeats in CRISPR regions of bacterial genomes are made up of sequences from
- Human DNA
- Bacteriophages
- Plant viruses
-Bacterial toxins

Answer 2

Bacteriophages

Question 3

The CRISPR system is found in — and uses — and Cas to destroy DNA
-Mammalian cells; RNAi
- Bacteria; RNAi
-mammalian cells; zinc-finger nucleases
-bacteria; guide RNAs

Answer 3

bacteria; guide RNAs

Question 4

Class I CAS proteins capture DNA that the cell has not previously encountered
-T/F

Answer 4

True

Question 5

In CRISPR technology, homology-directed repair is applied when attempting to replace a faulty gene - T/F

Answer 5

True

Question 6

PAM is an acronym for
- Protein - Adjustable marker
- Proroalternative motif
- Protospacer adjacent motif
- Protein adjustable mutation

Answer 6

Protospacer adjacent motif

Question 7

crRNA refers to as?
- Coliphage resistant RNA
- complementary RNA
-complementary ribosomal RNA
-CRISPR RNA

Answer 7

crispr RNA

Question 8

PAM recognition site is…
- GNC
-ACG
-NGG
-ATG

Answer 8

NGG

Question 9

A gene-editing technique was used to create a disease model for Rett Syndrome in maxques by altering the MECP2 gene. Which of the following methods would be considered the easiest and least costly to undertake this?
-TALENS
-UV mutagens
-CRISPR
-Zinc Finger

Answer 9

CRISPR