PCR EFFICENCY

Question 1

If not using a scientific calculator, to calculate “n” in the formula A = A. x PR
I should change the formula around as follows:
Select one:
(log(A/A.) )/logP= n
O n=logA+P+/ogA.
O n=logA + PlogA.
** n=A+ PlogAo

Answer 1

(log(A/A.) )/logP= n

Question 2

A bp is a loose term that molecular biologist use to describe a
Select one:
- a nucleotide on one strand of double -stranded DNA that is hydrogen bonded
to a nucleotide on the complementary strand
* a nucleotide beside another nucleotide on a single strand of DNA
* a base on one strand of double -stranded DNA that is hydrogen bonded to a base on the complementary strand
-two bases that are next to each other along a single strand of DNA

Answer 2

a nucleotide on one strand of double -stranded DNA that is hydrogen bonded
to a nucleotide on the complementary strand

Question 3

If a single molecule of water weighs 18 Daltons, then a mole of water weighs
Select one:
* 18 Daltons
* 18 Grams
6.023 x 1023 ug
* 6.023 x 1023 Grams

Answer 3

18 grams

Question 4

A single nucleotide weighs
Select one:
* 660 Daltons
* 330 Daltons
* 660 fa
330 pg

Answer 4

330 daltons

Question 5

When PCR efficiency is low, the PC amplicon..
Select one:
* will still form a visible band on a gel
* will not form a visible band on a gel
-will run off the gel ahead of the tracking dye
* may not form a visible band on a gel

Answer 5

• may not form a visible band on a gel

Question 6

What does “A.” represent in the Formula:
A = A. x PR
Select one:
* Cycle Number
* Efficiency of the PCR reaction
* Yield after PCR
* Starting amount of amplicon pre PCR

Answer 6

• Starting amount of amplicon pre PCR

Question 7

What does “A” represent in the Formula:
A = A. x PR
Select one:
* Starting amount of amplicon pre PR
* Cycle Number
* Efficiency of the PC reaction
* Yield after PCR

Answer 7

• Yield after PCR

Question 8

The molecular weight of an individual atom or an individual molecule is described using which weight unit
Select one:
⁃ Grams
⁃ ug
⁃ Daltons
⁃ ng

Answer 8

daltons

Question 9

What does “n” represent in the Formula:
A = A. x Pr
Select one:
⁃ Starting amount of amplicon pre PCR
⁃ Yield after PCR
⁃ Cycle Number
⁃ Efficiency of the PCR reaction

Answer 9

cycle number

Question 10

What does “P” represent in the Formula:
A = A. X PR
Select one:
⁃ Cycle Number
⁃ Starting amount of amplicon pre PCR
⁃ Efficiency of the PCR reaction
⁃ Yield after PCR

Answer 10

efficiency of PCR reaction