Bacterial Replication

Question 1

1. A lagging strand of DNA is synthesized discontinuously.
   True
   False

Answer 1

True

Question 2

The DNA lagging strand is made entirely using DNA polymerase I
True
False

Answer 2

False

Question 3

DNA is synthesized during
Prophase
Metaphase
Anaphase
None of the above

Answer 3

None of the above

Question 4

The gaps between Okazaki fragments are
Filled by DNA polymerase III
Repaired by DNA polymerase I
Repaired by DNA polymerase III
Filled in by DNA polymerase I

Answer 4

Filled in by DNA polymerase I

Question 5

In bacterial DNA synthesis the lagging strand is made 5 minutes after the leading strand
True
False
I do not know

Answer 5

false

Question 6

Natural primers are made of
Double-stranded DNA
A short string of amino acids
Single-stranded RNA
Single-stranded DNA

Answer 6

single stranded RNA

Question 7

During replication DNA gyrase
Links the 3’-OH and 5’-PO4 of neighbouring nucleotides
Removes the supercoiling
Arranges nucleotides on the antisense strand
Synthesize short primers

Answer 7

Removes the supercoiling

Question 8

Bacterial chromosomes are usually circular, and replication therefore proceeds in
One direction around the circle
Two directions around the circle
Three directions around the circle
Four directions around the circle

Answer 8

Two directions around the circle

Question 9

Single stranded binding protein
Primase
DNA polymerase
DNA gyrase
DNA ligase

Answer 9

Single stranded binding protein
➢ Keeps the two complementary strands of DNA separated
Primase
➢ RNA polymerase used to synthesize short RNA primers
DNA polymerase
➢ Processively adds a complementary nucleotide to the 3’ end of a new strand of
DNA
DNA gyrase
➢ Untwists strands of DNA
DNA ligase
➢ Links the 3’-OH to the 5’-PO4 forming the phosphodiester bridge

Question 10

In bacterial DNA replication, most of the DNA is synthesized by the enzyme
DNA Polymerase I
DNA Polymerase II
DNA Polymerase III
DNA Taq Polymerase

Answer 10

DNA polymerase III