PCR Practical

Question 1

A PCR master mix can be divided into aliquots and should never be refrozen- T/F

Answer 1

True

Question 2

In a single PCR cycle consisting of 30 seconds at 95 degrees, 1 min at 55 C, 1 min @ 72C what is happening at 55C
- The DNA to be amplified is being denatures
- Primers are being denatured
-DNA polymerase is extending new DNA from the primers
- Primers are annealing to DNA

Answer 2

• Primers are annealing to DNA

Question 3

Commercial PCR mixes containing magnesisum usually include it tp for a final concentration reaction tube of ?
- 8mM
-12mM
-7mM
-1.5-2mM

Answer 3

1.5-2mM

Question 4

Taq polymerase units added to a PCR reaction are usually in the range
-3-5U
-1.5-2.U
-2-4U
-4-5.6U

Answer 4

1-2U

Question 5

All of the following are required for PCR except…,
- Polymerase to synthesize DNA
-DNA gyrase to unwind DNA
- 2 primers to direct DNA amplifixation
- dNTPS, the building blocks of DNA
- All are required for PCR

Answer 5

• DNA gyrase to unwind DNA

Question 6

The typical concentration of each dNTPS is 50micromoles and together al four should have a collective concentration of 200 micromoles

Answer 6

True