RNA Transcription

Question 1

At what site of the growing chain are nucleotides added?

Answer 1

3´OH of the growing end

Question 2

What is the direction of new chain synthesis?

Answer 2

5´ –> 3´

Question 3

In which direction is the template copied?

Answer 3

from 3´to 5´

Question 4

How is transcription initiated?

Answer 4

Multiple transcription factors -> complex formation on promoter region -> positioning of RNA Pol II on +1 on starting site

Question 5

How is transcription controlled and what is needed?

Answer 5

several nt sequences, co-factors, RNA Pol II, transcription factors

Question 6

What are the AAs on the C-terminal domain of RNA Pol II?
How is it controlled?

Answer 6

YSPTSPS repeated 52 times in mammalian Pol II
Phosphorylated when transcribing DNA

Question 7

Where is the promoter of the human growth hormone (GH) gene located?

Answer 7

Chromosome 17

Question 8

Describe GH transcription factor:

Answer 8

Pit-1, it has a DNA binding domain, and a transcriptional-activation domain to activate RNA Pol II

Question 9

What are known mutations in Pit-1 and what are the effects?

Answer 9

E174G and R172X

Pit-1 doesn’t bind DNA -> no transcription of GH -> short stature