RNA Metabolism

Question 1

How many RNA polymerase do bacteria have?

Answer 1

One

Question 2

What is the DNA regulatory element involved in the initiation of transcription called?

Answer 2

The promoter

Question 3

A promoter is a mosaic of?

Answer 3

Consensus sequences and non-consensus sequences

Question 4

What determines the strength of the promoter?

Answer 4

Consensus sequences, the spacing between them, and distance to the transcription start site

Question 5

The transcription bubble extends over ______ DNA base pairs and the RNA/DNA hybrid is about ___ bps

Answer 5

15-17, 7

Question 6

Termination of transcription is regulated by which specific sequences?

Answer 6

GC rich region in combination with AU or CA rich region

Question 7

The antibiotic Rifampin inhibits initiation of transcription by?

Answer 7

Binding to RNA polymerase

Question 8

The antibiotic Actinomycin inhibits transcription elongation by?

Answer 8

Binding to template DNA

Question 9

The binding of eukaryotic RNA _______________ to the promoter is preceded by the binding of which general transcription factors to the promoter?

Answer 9

Polymerase II, TFIIB and TFIID/TBP

Question 10

Polyadenylation is the replacement of the ____ end of the ____________ mRNA by a poly-a stretch

Answer 10

3’ end, nascent mRNA

Question 11

Splicing is the removal of?

Answer 11

Introns

Question 12

What are the three sources for variant mRNA?

Answer 12

Alternate start sites, alternative splicing, alternative termination sites

Question 13

Shortening of poly-a tail beyond ____ a’s leads to _____________ of the mRNA

Answer 13

25, degradation

Question 14

The DNA coding strand is ___________ to the mRNA transcribed from the template strand

Answer 14

Identical