Protein Metabolism

Question 1

The large bacterial ribosomal subunit is ______, and consists of ____ rRNA, ____ rRNA and 36 proteins

Answer 1

50S, 23S, 5S

Question 2

Eukaryotic ribosomes carry an extra _____ rRNA subunit in the ________ subunit

Answer 2

5S, large subunit

Question 3

There are _____ codons in the genetic code, that code for _____ amino acids, and ____ different stop codons

Answer 3

64, 20, 3

Question 4

Any insertion or deletion that is not 3n or a multiple of 3 will cause?

Answer 4

Frame shift mutation

Question 5

Inside in the 5’ anticodon position can recognize which codons in tRNA?

Answer 5

A, U, C

Question 6

Anticodon U in the 5’ position can recognize which codons of tRNA?

Answer 6

A, G

Question 7

Anticodon G in 5’ position can recognize what codons of tRNA?

Answer 7

C, U

Question 8

Misses mutations cause the codon to code for?

Answer 8

Another amino acid

Question 9

Nonsense mutation results in?

Answer 9

Stop codon = protein termination

Question 10

Activation of amino acid takes place _________ tRNA is charged

Answer 10

Before

Question 11

The first amino acid incorporated is usually? How is it usually found in bacteria?

Answer 11

Methionine, usually formylated in bacteria

Question 12

The Shine-Delgarno sequence makes connection between _____________ and _______ ___________, as well as ______ and _________

Answer 12

Bacterial mRNA and small ribosomal subunit, start codon and P-site

Question 13

What is the role of EF-Tu?

Answer 13

Direct charged tRNA to the A-site for protein elongation at the expense of hydrolysis of one high energy phosphate bond

Question 14

What does recycling if EF-Tu mean?

Answer 14

Charged with GTP with the help of Ts protein (replaces GDP with GTP)

Question 15

Formation of the peptide bond in bacteria is catalyzed by ____ rRNA and by ____ rRNA in eukaryotes

Answer 15

23S, 28S

Question 16

In protein elongation, 4 high-energy bonds are consumed from?

Answer 16

2 from 1 ATP molecule, and 2 from 2 GTP molecules

Question 17

Resistance to erythromycin is mediated by plasmid-borne RNA methylase that?

Answer 17

Methylates a specific A-site in the 23S rRNA

Question 18

Hsp70 chaperone is working ______________, while Hsp60 uses a _______________

Answer 18

Co-translationally, folding chamber

Question 19

Hydroxylated proline is an important constituent of _________________

Answer 19

Fibrillar collagen molecules

Question 20

Addition of fatty acids to amino terminals or cysteine groups do what?

Answer 20

Anchor proteins to membranes

Question 21

Ubiquination usually occurs on _____________ and means?

Answer 21

Lysine residues, sent to proteasome for degradation

Question 22

Misfortune of proteins results in?

Answer 22

Conversion of all secondary structures into beta sheets which stack above one another

Question 23

The small ribosomal subunit is ______, and consists of ______ rRNA and 21 proteins

Answer 23

30S, 16S