Rheumatoid

Question 1

is a suspension of polystyrene latex particles sensitized
with specially prepared human IgG.

Answer 1

Rheumatoid factor latex agglutination test

Question 2

The RF reagent is a suspension of __________________ sensitized
with specially prepared human IgG.

Answer 2

polystyrene latex particles

Question 3

The RF reagent is a suspension of polystyrene latex particles sensitized
with specially prepared human __________.

Answer 3

IgG

Question 4

The ______________________has been adjusted to detect a minimum of 8 IU/mL of rheumatoid factors according with the WHO International
Standard without previous sample dilution.

Answer 4

RF latex reagent sensitivity

Question 5

The RF latex reagent sensitivity has been adjusted to detect a minimum
of _____ IU/mL of rheumatoid factors according with the WHO International
Standard without previous sample dilution.

Answer 5

8

Question 6

The RF latex reagent sensitivity has been adjusted to detect a minimum
of 8 IU/mL of rheumatoid factors according with the ___________________without previous sample dilution.

Answer 6

WHO International Standard

Question 7

A suspension of uniform polystyrene
particles coated with IgG (human) in glycine buffer, pH 8.2;
reagent sensitivity is standardized with the World Health
Organization RF Standard.

Answer 7

Rheumatoid factor latex agglutination

Question 8

Principle of rheumatoid factor latex agglutination test

Answer 8

A suspension of uniform polystyrene
particles coated with IgG (human) in glycine buffer

Question 9

RF Latex Reagent: A suspension of uniform polystyrene
particles coated with IgG (human) in __________ buffer, pH 8.2;
reagent sensitivity is standardized with the World Health
Organization RF Standard.

Answer 9

glycine

Question 10

. RF Latex Reagent: A suspension of uniform polystyrene
particles coated with IgG (human) in glycine buffer, pH ________;
reagent sensitivity is standardized with the World Health
Organization RF Standard.

Answer 10

8.2

Question 11

RF Positive Control Serum: A stabilized, prediluted human
serum containing at least _______ IU/mL of RF.

Answer 11

at least 8

Question 12

A stabilized, prediluted human
serum containing at least 8 IU/mL of RF.

Answer 12

Rheumatoid factor positive control serum

Question 13

RF Negative Control Serum: A stabilized, prediluted human
serum containing less than ______ IU/mL of RF.

Answer 13

less than 8

Question 14

A stabilized, prediluted human
serum containing less than 8 IU/mL of RF

Answer 14

Rheumatoid factor negative control serum

Question 15

Glycine-Saline Buffer (20x): component

Answer 15

pH 8.2 ± 0.1M glycine and 0.15M
NaCl

Question 16

Buffer used

Answer 16

Glycine-saline buffer (20x)

Question 17

Sensitivity of rheumatoid factor latex agglutination test

Answer 17

8 IU/mL

Question 18

Procedure methods

Answer 18

Quantitative method and semi-quantitative method

Question 19

can be performed in the same way as the qualitative test using dilutions of the
serum in saline, phosphate buffer saline or glycine saline

Answer 19

Semi-quantitative determination

Question 20

Normal levels (adults): semi-quantitative method

Answer 20

less than 8 IU/ml

Question 21

is expressed as the reciprocal of the highest dilution
showing macroscopic agglutination.

Answer 21

Titer

Question 22

Qualitative Test: negative result

Answer 22

uniform milky suspension with no agglutination

Question 23

Qualitative Test: positive results

Answer 23

any observable agglutination

Question 24

Semi-quantitative method An estimateof the RF concentration in the specimen can be
expressed in

Answer 24

IU/mL

Question 25

Women are three times as likely to be affected as
men; in addition, the prevalence of the disease is highest in women who are
more than

Answer 25

65 years old

Question 26

RA can be characterized as a chronic, symmetric, and erosive arthritis of
the peripheral joints that can also affect multiple organs such as the

Answer 26

Heart and lungs

Question 27

The strongest associations have been between a subsetof
patients with RA and

Answer 27

HLA-DRB 1 alleles or PTPN22 gene polymorphism

Question 28

The strongest associations have been between a subsetof
patients with RA and

Answer 28

HLA-DRB 1 alleles or PTPN22 gene polymorphism

Question 29

The strongest
environmental risk factor for RA is believed to be

Answer 29

Cigarette smoking

Question 30

The pathology of RA is caused by an

Answer 30

Inflammatory (bone and cartilage)

Question 31

The pathology of RA is caused by an

Answer 31

Inflammatory (bone and cartilage)

Question 32

Infiltration of the inflamed synovium with _____________,___________, dendritic cells,_____________,and granulocytes is evidence of immunologic activity within the joint.

Answer 32

T and B lymphocytes
plasma cells

mast cells

Question 33

Two key antibodies found in the disease are

Answer 33

Rheumatoid factor and anti cyclic citrullinated peptide

Question 34

Rf is an antibody that is most often of the immunoglobulin

Answer 34

IgM class

Question 35

RA, autoantibodies such as RF and anti-CCP are thought to combine with
their specified antigen, and the resulting immune complexes become
deposited in the joints, resulting in a

Answer 35

type III (or immune complex)
hypersensitivity reaction.

Question 36

RF can be found in patients with

Answer 36

Systemic lupus erythematosus
Mixed connective tissue disease
Scleroderma
Sjogren’s syndrome

Question 37

using charcoal or latex
particles coated with IgG have been used for many years
to detect RF

Answer 37

Manual agglutination test

Question 38

These tests, however, only detect the IgM isotype, found
in about 75% of patients, and have been largely replaced
by

Answer 38

enzyme-linked immunosorbent assay.
chemiluminescence immunoassay,
nephelometric methods

Question 39

Once a diagnosis of RA is made, the most helpful tests
for following the progress of the disease are general
indicators of inflammation, such as measurement of

Answer 39

C reactive protein
Erythrocyte sedimentation rate
Complement component

Question 40

are elevated and the levels of
serum complement components are normal or
increased because of increased acute-phase reactivity.
CRP levels correlate well with disease activity because
levels reflect the intensity of the inflammatory
response.

Answer 40

Erythrocytes sedimentation rate
C reactive protien

Question 41

Increased levels of RF may be round in some
diseases other than rheumatoid arthritis such as

Answer 41

Sarcodosis, sle and sjogren’s syndromes

Question 42

One of the most useful clinical markers for rheumatoid
arthritis is

Answer 42

Rheumatoid factor in serum

Question 43

Rheumatoid factor is a term used to describe a variety of ____________ or __________or both,

Answer 43

antibodies or immune complexes

Question 44

Reagents are stable until stated expiration date on
bottle label when stored refrigerated

Answer 44

2-8C