NON TREP Flashcards
Non-Treponemal
test for Syphilis especially
Rapid plasma reagin
Rapid Plasma Reagin (RPR) specimen of choice
Serum
Rapid Plasma Reagin (RPR) using
patient serum mixed with
RPR carbon antigen
RPR POSITIVE RESULTS
Flocculation
is a contagious sexually transmitted disease cause
by the spirochete Treponema pallidum
Syphilis
Syphilis is a contagious sexually transmitted disease cause
by the spirochete
Treponema pallidum
This spirochete causes an
immune response in the body by producing antibody-like substance
in plasma or serum known as
Reagin
derived from the beef heart reacts with the reagin and this
antigenic property of cardiolipin is used in non-treponemal test.
Cardiolipin and phospholipid
is the most frequently performed non treponemal test for
syphilis
Rapid plasma reagin
The specimen serum or plasma is mixed with the RPR card
antigen suspension and allowed to react for
8 mins
If reagin is present, it will react to the antigen suspension
forming visible
Black floccules
Rapid plasma reagin This result is result is reported a
Reactive
If reagin is absent, there will be no flocculation. This result
is reported as
Non reactive
Hold the dropper over a test card circle and squeeze the teat to allow one drop or in ul
50 ul
Reactive result indicate as
Flocculation