Ana Flashcards
Principle of anti nuclear antibodies
Enzyme linked immunosorbent assay
Chemiluminescence immunoassay
are assays that can test from a broad range of
antibodies if multiple nuclear antigens are coated onto a single test
well,
Elisa and clia
Reagent used in qualitative
Sle latex reagent
Positive Result of ana
Agglutination
Negative result of ana
No agglutination
POSITIVE sera
must show distinct agglutination within
3 mins
Agglutination indicates the level of antinuclear antibody
(especially
Anti DNP
recommended that the IIF
test remains the gold standard for ANA testing
American College of Rheumatology
Acr recommended that the _____remains the gold standard for ANA testing
Indirect immunofluorescent
is a chronic systemic inflammatory
disease that affects people usually at the peak age between 20 and 40
years
Systemic lupus erythematosus
Systemic Lupus Erythematosus is a chronic systemic inflammatory
disease that affects people usually at the peak age between
20 and 40 years
Women are much likely more to be affected than men by a ratio of
9:1
This autoimmune disease appears to originate from complex interaction
between several factors, such as
Environmental, genetic and abnormalities in immune system
associated with SLE have
been discovered.
100 autoantibodies
These include antibodies to double stranded DNA (dsDNA),
histones, and other nuclear components, as well as
Lymphocyte
Erythrocytes
Platelets
Phospholipids
Endothelium
Ribosomal component
Laboratory diagnosis of SLE include
Complete blood count
Platelet count
Urinalysis
Findings in lupus patients are
Leukopenia, anemia, thrombocytopenia
Test may be elevated result of sle
Erythrocyte sedimentation rate
Test tends to be low
or normal. Sle result
C reactive protein
the first test typically done is a screening test for sle
Anti nuclear antibody
They can also be found in some individuals with other conditions, including
chronic infections, pregnancy, and cancer
Possible result for IIF TESTING
Speckle
Homogenous
Rim
Nucleolar
Centromere
CREST MEANS
Calcinosis
Raynaud phenomenon
Esophageal dysfunction
Sclerodactyly
Telanglectasias
calcium deposits in the skin
Calcinosis
spasm of blood vessels in response to cold or stress
Raynaud’s phenomenon
acid reflux and decrease in motility of esophagus
Esophageal dysfunction
thickening and tightening of the skin on the fingers and hands
Sclerodactyly
dilation of capillaries causing red marks on surface of skin
Telanglectasias
