rh group Flashcards
who described a hemolytic transfusion reaction in an obstetrical patient.
Levine and Stetson
two tests under HEMOGLOBIN F DETERMINATION
- Alkali denaturation test
> Betke test (sodium hydroxide)
> Singer test (potassium hydroxide) - Acid Elution test
> Kleihauer-betke test
Given to pregnant women that is capable of
producing anti-D when exposed to D positive
RBCs.
Rh IMMUNE GLOBULIN (rhogam)
what is the principle of rhogam
to prevent immunization to D antigen by the use of high titer RhIg
if POSITIVE: FMH
must be quantified using the Kleihauer-Betke test
If NEGATIVE in PH
the mother should
receive a full dose of RhIg within 72
hours of delivery.
used during
pregnancy to quantify the amount of
fetal blood found in the maternal
circulation
kleihauer-betke test
a postpartum blood sample is drawn from the
mother
postpartum dosage
Should be given to nonimmunized D-negative
females at 28th weeks’ gestation
ANTEPARTUM DOSE (300 μg anti-D IM or IV)
is advised after 34th weeks’ gestation when
amniocentesis is performed or in the event of
obstetric complication or following termination of
pregnancy
120 ug dose rhogam
FULL DOSE RHOGAM
protect 30 ml of WB and 15 ml RBC
MINI OR MICRODOSE RHOGAM
protects up to 5 ml D + WB 2.5 ml D + RBC
Given after 12 weeks of pregnancy (Rh negative) to
avoid abortions and ectopic pregnancies,
miscarriage.
full dose rhogam
given to the first 12 weeks of pregnancy (Rh
negative) for abortions and ectopic pregnancies,
miscarriage
mini or microdose rhogam
five antigens made up the Rh
system.
D > c > E > C > e
HDN will lead to
death, spontaneous abortion, ectopic pregnancy, miscarriage
Reported on an antibody made by guinea
pigs and rabbits when they were transfused
with rhesus monkey RBCs
Landsteiner and Wiener
anti-rhesus antibody formed by the
animals was renamed
anti-LW
Based on the theory that the antigen of the
systems were produced by the three closely
linked set of alleles, each gene was responsible
for producing a product (or an antigen) on the red cell membrane.
FISCHER-RACE (DCE)
Postulated that the gene responsible for defining
Rh are actually produced an agglutinogen that
contains a series of blood factors, in which each
factor is an antigen recognized by an antibody
WIENER (Rh-Hr)
Number is assigned to each antigen of the Rh
system in order of its discovery
ROSENFIELD (ALPHA NUMERIC)
The two most important nomenclature are
Fischer/Race and Wiener nomenclature
only describes the presence or
absence of a given antigen
Rosenfield
absence of the capital D antigen
small d
