compatibility testing Flashcards

1
Q

most common cause of error in crossmatching

A

misidentification of the recipient

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2
Q

content of requisition form

A

patients full name and unique hospital identification number\
age, birthday, name, address, sex and
requesting physician

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3
Q

agglutination or hemolysis means

A

incompatible sample

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4
Q

example of in vivo hemolytic processes

A

hemolytic anemia

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5
Q

may cause small fibrin clot to form and may
be difficult to distinguish from true agglutination

A

plasma

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6
Q

Patients with IV tubing lines:

A

Extraction should be done below the IV line

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7
Q

Both donor and recipient samples must be stored for

A

minimum of 7 days

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8
Q

Three phases of crossmatching:

A

Immediate Spin Phase
Incubation phase or the thermo phase
▪AHG phase or Coomb’s phase

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9
Q

must be performed on a sample of blood taken at the time of
collection from the donor.

A
  • ABO
  • Rh typing
  • Weak D determination
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10
Q

sample used for testing must be obtained from

A

attached segment - hermetic seal

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11
Q

These can be performed in advance of or at the same time
as the crossmatch.

A

ABO, Rh grouping, and antibody screening

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12
Q

If the Rh type of the recipient cannot be determined and
transfusion is essential

A

Rh negative blood should be given

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13
Q

This test is unnecessary when testing
transfusion recipients

A

test for weak D

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14
Q

During emergency the only blood you could give is

A

packed red blood cell

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15
Q

Rh positive patient may receive

A

Rh positve and Rh negative

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16
Q

objective of the antibody screening test.

A

to detect clinically significant unexpected antibodies

17
Q

Presence of unexpected alloantibodies

A

▪ Allogenic transfusion of RBCs
▪ Pregnancy or transplantation/ organ tissue
transplantation
* Graft-rejection

18
Q

ANTIBODY SCREENING METHOD

A
  • Tube method
  • Gel-tech
  • Solid-phase technique
19
Q

ANTIBODY SCREENING - CROSSMATCHING

A

o AHG reagent
o Coomb’s check cell
o Sample of choice

20
Q

REACTION MEDIUM

A

22% Bovine Albumin
LISS
PEG

21
Q

gel used in gel technique

A

dextran acrylamide gel

22
Q

does not contain any specific reagent
and acts only by its property of trapping
agglutinates

A

Neutral

23
Q

use a specific reagent
incorporated into the gel and are useful for
antigen determination.

A

Specific gel tests

24
Q

valuable application of the gel
test and may be used for the IAT or the DAT.

A

AHG test

25
Q
A