RH Blood Group System

Question 1

ROUTINE RH TYPING
Detects:_______
Specimen:____________
Rgt:________
Result:_____

Answer 1

Detects: D antigen
Specimen: Whole Blood
Rgt: AHG Anti-B (antisera)
Result: Agglutination - Rh negative
No agglu - Rh positive

Question 2

WEAK D TYPING (Du)
A form of: _________
Specimen: __________
Rgt: _________
Result: _________

Answer 2

A form of: IAT
Specimen: whole blood / RCS( neg control: bovine albumin)
Rgt: AHG
Result: Agglu- weak D pos reported as Rh pos
No Agglu- Rh neg

Question 3

Blood recipients with the C trans weak D, genetic weak D have complete Ag and cannot reproduce allo-anti D. What can be transfused?

Answer 3

Rh positive blood

Question 4

All Rh negative, weak D negative obstetric patients are candidates for _____

Answer 4

Rh immune globulin

Question 5

It si a purified Anti D prepared from immunized donors and GIVEN TO D- NEGATIVE MOTHERS to prevent formation of anti-D

Answer 5

RhIg ( RhoGAM)

Question 6

Lacks ALL Rh antigen on their RBCs

Answer 6

Rhnull syndrome

Question 7

A mutation on RHAG gene and can pass through normal genes of children

Answer 7

Regulator type Rhnull syndrome

Question 8

Mutation in each RHCE gene and the common deletion of RHD gene

Answer 8

Amorphic type Rhnull syndrome

Question 9

Partial suppression of RH gene expression caused by mutations of RHAG gene

Answer 9

Rhmod phenotype

Question 10

Differentiation of ABO and RH

Question 11

Reaction phase of RH

Answer 11

37C at IAT PHASE

Question 12

Antigen composition of Rh blood group

Answer 12

Nonglycosylated proteins

Question 13

Antibody produce by injecting rehuses monkey rbc’s I to guinea pigs and rabbits, having the same specificity as the antibody Levine and Stetson decribed

Answer 13

Anti-LW

Question 14

Reacts:
strongly - D- positive RBCs
Weakly - Rh neg RBCs
Never - Rhnull cells

Answer 14

Anti-LW

Question 15

Most immunogenic Rh antigen.

Answer 15

D

Most immunogenic :D>c>E>C>e: least

Question 16

Present of 85% of whites, and a higher percentage of blacks, American, indians and Asians.

Answer 16

D antigens

Question 17

A nomenclature that based on postulated genetic mechanism of Rh antigen

Answer 17

Fisher race / DCE and Wiener /Rh-Hr

Question 18

A nomenclature that describes the presence or absence of antigen

Answer 18

Rosenfield/ Alphanumeric terminology

Question 19

Antigens were produced by THREE CLOSELY LINKED SETS OF ALLELES

Answer 19

Fisher Race/ DCE terminology

Question 20

Codes for the presence or absence of D antigen. Located at chromosome 1.

Answer 20

RHD

Question 21

Codes for the expression of Cc andEe antigens. Located at chromosome 1.

Answer 21

RHCE

Question 22

Produced Rh associated glycoprotein.
Considered as CO-EXORESSOR.

Answer 22

RHAG

Question 23

Denotes absence of D

Answer 23

d

Question 24

Postulated that Rh gene produced AGGLUTINOGEN which contains AT LEAST 3 BLOOD FACTORS, each factor an antigen.

Answer 24

Wiener:Rh-Hr terminology

Question 25

A substance that stimulates production of agglutinin thereby acting as an antigen.

Answer 25

AGGLUTINOGEN

Question 26

Complement of genes inherited form either parents

Answer 26

HAPLOTYPE

Question 27

Gene association: Dce - DCe - DcE - DCE - dce - dCe - dcE - dCE -

Answer 27

Dce - Rh0 DCe - Rh1 DcE - Rh2 DCE - Rhz dce - rh dCe - rh' dcE - rh'' dCE - rhy

Question 28

System that assigns number to each antigen. No genetic basis.

Answer 28

Rosenfield/Alphanumeric terminology

Question 29

Adopted a six digit number for each authenticated antigen binding to a blood group system

Answer 29

International society of blood transfusion committee: Updated numeric terminology

Question 30

Haplotype frequency in black, white, and Asians.

Answer 30

Black: Ro>r>R1>R2 White: R1>r>R2>RD Asians: R1>r>R2>Ro

Question 31

Importance of Rh terminologies

Answer 31

- parentage studies - predicting potential of HDN

Question 32

Main function of Rh antigen.

Answer 32

-Transport of ammonia and carbon dioxide -maintain the structural integrity of RBC membrane

Question 33

The greatest number of D antigen sites are on cells of the rare Rh phenotype of ________

Answer 33

D --

Question 34

Carry only D antigen and completely lack C c and E e

Answer 34

D --

Question 35

Cells posses the LARGEST NUMBER OF D antigen sites

Answer 35

R2R2

Question 36

Most common D positive genotype

Answer 36

R1r

Question 37

Most common D negative genotype

Answer 37

rr

Question 38

Inheritance of RHD genes that code for a WEAKENED EXPRESSION OF D ANTIGEN. Seen frequently in BLACK PERSONS

Answer 38

Genetic Weak D

Question 39

Position effect or gene interaction effect. They can receive D positive RBC'S with no adverse effects.

Answer 39

C in trans to RHD

Question 40

D antigen expression can be weakened when ONE OR MORE D EPITOPES WITHIN THE ENTIRE PROTEIN IS MISSING OR ALTERED

Answer 40

Partial D

Question 41

Extremely low number of D antigen sites . Adsorbing and eluting anti- D from individuals red cells. Relatively common on Asians

Answer 41

Del

Question 42

Type positive with anti-D even though the D epitope is on the RHCE protein.

Answer 42

D EPITOPES on RhCE protein

Question 43

Results from a hybrid gene RHCE-RHD-RHCE where only a small portion of RHD is on RHCE protein

Answer 43

DHAR

Question 44

African descent; amino acid change in the RHce gene

Answer 44

ceCF ( Crawford)

Question 45

Identify the detection method of the following: Genetic weak D: _________ C in trans to RHD: _______ Partial D: _______

Answer 45

Genetic weak D: Weak D test C in trans to RHD: Monoclonal reagents Partial D: Weak D or Monoclonal