Review Flashcards
1
Q
what kind of a virus is HIV?
A
a retrovirus with an RNA genome
2
Q
what is the role of a denaturing agent in gel electrophoresis?
A
disrupts hydrogen bonds and other interactions (i.e. natural structure is disturbed) causing it to unfold into a linear chain
3
Q
what is the role of a reducing agent in gel electrophoresis?
A
disrupts disulfide bonds
4
Q
what is the role of native state gel electrophoresis?
A
proteins retain their conformations
5
Q
what is k cat
A
number of substrate molecules each enzyme converts to product per unit time (turnover number)
6
Q
how do you find the efficiency of an enzyme?
A
k cat/Km
7
Q
k 2 is also known as
A
k cat
8
Q
V max =
A
k 2*[E total]
