Respiration Flashcards
What are the components of the matrix
Link reaction and krebs cycle occur.
Fluid like
Contains enzymes - for reactions - coenzymes
Mitochondrial DNA and ribosomes - to synthesise enzymes
What are the components of the outer membrane
protein channels and carriers present
phospholipid membrane
What are the components of the inner membrane
phospholipid bilayer which is less permeable to small ions
cristae - highly folded to increase SA
electron carriers- electron transport chain
What are the significance of the intermembrane space’s location
close contact with the matrix to allow for reduces NAD and FAD to deliver protons to the electron transport chain
Why may mitochondria look different in microscope images
cut across different planes of the mitochondria
Name the order of the stages of respiration
and where does each stage occur
- glycolysis -cytoplasm
- link reaction -matrix
- Krebs cycle -matrix
- oxidative phosphorylation and chemiosmosis- cristae/matrix/im space
Explain the stages of glycolysis
- PHOSPHORYLATION 6
GLUCOSE is broken down by 2 ATP into HEXOSE1,6 BISPHOSPHATE
2.LYSIS 3
HEXOSE 1,6 BISPHOSPHATE breaks down into 2 TRIOSE PHOSPHATE MOLECULES.
3.OXIDATION 3
EACH TRIOSE PHOSPHATE breaks down into PYRUVATE by 2 ATP and NAD gains 2H+ becoming REDUCED NAD
4.PYRUVATE is ACTIVELY TRANSPORTED to the LINK REACTION
Explain the stages of the Link reaction
PROCESS HAPPENS TWICE
- PYURVATE gets to the MATRIX through the outer membrane via a protein SYMPORT via ACTIVE TRANSPORT
- a CARBON gets REMOVED from PYRUVATE (decarboxylated)- then loses a H+ to NAD ( becomes reduced NAD via OXIDATION) making ACETATE
3.ACETATE combines with COENZYME A to become ACETYL COENZYME A
Explain the stages of the Krebs cycle
PROCESS HAPPENS TWICE
- ACETYL GROUP reacts with OXALOECAETAE making CITRATE
2carbon + 4carbon = 6 carbon - CARBON DIOXIDE is removed (DECARBOXYLATED) H+ and e- are removed and reduced NAD (DEHYDROGENATED) makes a 5 carbon compound. CARBON DIOXIDE is removed from the 5 C compound making a 4 C compound
6C - co2 = 5C - co2 = 4C - H+ and e- are REMOVED forming REDUCED NAD.
ATP is made via SUBSTRATE LEVEL PHOSPHYRLATION (without a membrane)
H+ and e- removed from FAD making REDUCED FAD
H+ and e- removed again from NAD forming REDUCED NAD
Explain the stages of Oxidative Phosphorylation.
- REDUCED NAD/FAD release PROTONS AND ELECTRONS into the ELECTRON TRANSPORT CHAIN via ELECTRON CARRIER PROTEINS
- ELECTRONS RELEASE ENERGY as they travel causing H+ to be ACTIVELY TRANSPORTED inti the IM SPACE from the MATRIX through the CRISTAE
3.INCREASED CONC of H+ in the IM SPACE - creating an ELECTROCHEMICAL GRADIENT.
- CHEMIOSMOSIS ( movement of protons from a high to low concentration) DOWN ELECTROCHEMICAL GRADIENT occurs INTO the MATRIX via ATP SYNTHASE.
5.Protons drive ATP PRODUCTION by supplying a PROTON MOTIVE FORCE. OXYGEN reacts with PROTONS and ELECTRONS to make WATER
How many ATP molecules can be made from 1 glucose molecule :
explain how
32
glycolysis = 7
link reaction = 5
krebs cycle = 19
Why arent 32 ATP molecules always made in aerobic respiration
ATP is used during the process of respiration :
actively transport pyruvate to mitochondrial matrix
used in ETC
ATP used to transport NAD to reduced NAD
What stage of respiration can occur in anaerobic respiration and why
Glycolysis - the only process which doesn’t require oxygen.
Why cant the krebs cycle, oxidative phosphorylation occur in anaerobic respiration
no final electron acceptor in the ETC oxidative phosphorylation
Reduced NAD and FAD aren’t oxidised by an electron carrier –> no NAD/FAD for dehydrogenation in the krebs cycle.
Describe the process of lactate fermentation
Pyruvate ( made from glycolysis) gets converted into LACTATE ( lactic acid).
Lactate dehydrogenase enzyme
Reduced NAD loses 2 electrons and becomes NAD (oxidised)