REQUIRED PRACTICALS

Question 1

Required practical 3:

Production of a dilution series of a solute to produce a calibration
curve with which to identify the water potential of plant tissue.

Answer 1

1)
> Make a series of dilutions of 1M sucrose solution ― at 0.0, 0.2, 0.4, 0.6, 0.8 and 1.0M sucrose.
> Dilute using distilled water.

2)
> Measure 5cm³ of each dilution into separate test tubes.

3)
> Use a cork borer to cut out 6 potato chips and cut them into identically sized chips.
> Dry each chip using a paper towel to remove excess water but do not squeeze.

4)
> Weigh each before the start of the experiment.

5)
> Place a potato chip in each test tube and leave for 20 minutes.

6)
> Remove each potato chip, dry gently using paper towel, and weigh them in turn.

7)
>Calculate the percentage change in mass for each sucrose solution

Question 2

What is 1M?

Answer 2

1 mol/dm³