ELISA Test Flashcards
1
Q
How is an ELISA Test carried out?
A
- Apply sample to surface (antigens attach)
- Wash to remove unattached antigens
- Add specific antibody + leave to bind
- Wash to remove excess antibody
- Add second antibody with enzyme attached to bind with first antibody.
- Add colourless enzyme substrate to change colour.
- Quantity = intensity