Required Practical: effect of PH on Amylase Flashcards
1
Q
Amylase is an enzyme that…
A
breaks down starch molecules into simply sugars - glucose
2
Q
Iodine turns ______ _______ from _______ when starch is present
A
blue - black from orange
3
Q
- Place one drop of ____ _______ into each well of a ______ _______
A
iodine solution
spotting tile
4
Q
- take three test tubes each with:
A
- 2cm3 of starch solution
- 2cm3 of amylase solution
- 2cm3 of pH 5 buffer solution
5
Q
what does buffer solution do?
A
controls pH of a solution - keeps it same
6
Q
- Place the three test tubes in a _____ ________ so that they are brought to the same temperature
A
water bath
7
Q
- combine the three solutions into one and return to __________ ________ whilst stirring with a ________ _____
A
water bath
stirring rod
8
Q
- every 30s add a drop of the solution to the….
A
wells with iodine solution in the spotting tile
9
Q
- repeat until…
A
iodine does not turn blue-black (stays orange) signifying that starch is no longer present i.e the reaction is complete and the amylase has broken down all of the starch
10
Q
- repeat using different
A
pH buffer solutions
11
Q
Conclusion:
A
the solution that causes the starch to be broken down quickest is the one that has the best pH for amylase to work -> fewest tiles gone blue-black.
