Required practical 9 - Respiration Flashcards
1
Q
Why are the tubes left for x minutes in the water bath before adding the methylene blue?
A
Allows the yeast / glucose mixture to equilibrate / reach the temperature of the water bath
2
Q
How would the temperature be kept constant?
A
thermostatically controlled water bath
3
Q
Why would you not shake the tube?
A
Oxygen would act as the final electron acceptor instead of methylene blue
4
Q
Explain how the end point for this practical is standardised
A
Set up a control to compare results to - yeast and glucose solution owith no methylene blue
5
Q
Suggest one source of error
A
Colour change end point is difficult to determine
