Required practical 2 - Mitosis Flashcards

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1
Q

Why is toluidine blue used?

A

Chromosomes are stained so are visible

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2
Q

Why is the tissue macerated?

A

To separate the cells

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3
Q

Why is the tissue squashed under a cover slip?

A

single layer of cells so light can pass through

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4
Q

Why should the student take care not to squash the cells sideways?

A

to avoid damage to the cells

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5
Q

Why are cells from the root tip used?

A

these are undergoing mitosis as plants grow at root and shoot tips

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6
Q

Why calculate percentage of cells undergoing mitosis rather than counting number of cells?

A

Different tissues may have different numbers of cells so results are more comparable

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