Required Practical

Question 1

Describe how to use aseptic techniques to transfer bacteria from bottle to agar plate (3)

Answer 1

• wash hands with SOAP and wipe surfaces with disinfectant
• use sterilised unopened pipette
• use sterile spreader

Question 2

Describe and explain 3 ways the scientist ensure he used aseptic techniques? (3)

Answer 2

• wash hands before and after experiment to remove microbes
• flame equipment and neck of bottle to kill microbes to prevent contamination
• life lid slightly at small angle to prevent entry of microbes of the plate