Refractometers And Blood Tubes Flashcards

0
Q

How does the refractometer work

A

By refraction: bending of light rays as they pass from a medium into another with a different optical density

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1
Q

What does the refractometer do

A

Measures protein concentration in a liquid, measures fluid specific gravity

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2
Q

What fluid types can you measure protein concentration from in the refractometer

A

Plasma, body fluids, cerebrospinal fluid, liquids accumulating in body cavities

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3
Q

What is the unit for protein concentration

A

In grams per 100 mL

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4
Q

What is the specific gravity based off of

A

Weight of a substance compared to water.

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5
Q

What are exudate

A

Fluids containing a high amount of protein and cellular debris escaped from blood vessels. Usually a result of inflammation

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6
Q

What are transUdates

A

Fluids escaped from tissues or blood vessels. Contain small amount of proteins or solids

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7
Q

What is the built-in prisim used for in the refractometer

A

Deviation and dispersion of light

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8
Q

What is special about the scale of the refractometer

A

It needs to be calibrated and tested. Measures specific gravity of urine and protein concentration in grams per 100 mL

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9
Q

What are the four components of the refractometer

A

Eyepiece, cover plate, prism, calibration screw

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10
Q

Describe the AO TS refractometer

A

Plasma protein and urine specific gravity. Measures refractive index, protein concentration of plasma, urine specific gravity.

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11
Q

What does a uricon refractometer measure

A

Urine specific gravity, refractive index scale

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12
Q

What does the Leica vet 360 refractometer measure

A

Urine specific gravity for dogs and cats as well as serum or plasma protein in grams per 100 mL

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13
Q

How do you use the refractometer

A

Please drop of sample on prison plus gently close cover plate. Look at scale through eyepiece. Read result.

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14
Q

How do you clean the refractometer

A

Wipe clean with tissue paper and distilled water

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15
Q

Why do you avoid any gritty material on the prism

A

So that it doesn’t scratch the lens

16
Q

How often should you calibrate the refractometer

A

Weekly

17
Q

With what fluid should you calibrate the refractometer

A

Distilled water. Scale should read zero

18
Q

How do you calibrate the refractometer

A

With the adjusting screw.

19
Q

Can you immerse the refractometer and water

A

No

20
Q

What do we do blood testing for

A

Complete blood, biochemistry, coagulation profile, immunology testing, blood bank testing, DNA testing

21
Q

When you do a biochem what are the components

A

Protein, glucose, lipids, electrolytes, enzymes, hormones

22
Q

What is plasma and what is it used for

A

Plasma is obtained after centrifugation of blood with a tube containing an anticoagulant such as EDTA, sodium site trait, heparin. Can be used for biochemistry analysis and total protein

23
Q

What are EDTA tubes used for

A

Plasma collection after centrifugation. Hematocrit determination by microhematocrit Capillary tube and a blood smear

24
Q

What is serum and what is it used for

A

Obtained after centrifugation of blood that has been allowed to coagulate. The clot composition is red blood cells white blood cells platelets fibrin and coagulation factors. Serum is used for blood biochemistry, red top tubes, tiger top tubes