Refractometers And Blood Tubes Flashcards
How does the refractometer work
By refraction: bending of light rays as they pass from a medium into another with a different optical density
What does the refractometer do
Measures protein concentration in a liquid, measures fluid specific gravity
What fluid types can you measure protein concentration from in the refractometer
Plasma, body fluids, cerebrospinal fluid, liquids accumulating in body cavities
What is the unit for protein concentration
In grams per 100 mL
What is the specific gravity based off of
Weight of a substance compared to water.
What are exudate
Fluids containing a high amount of protein and cellular debris escaped from blood vessels. Usually a result of inflammation
What are transUdates
Fluids escaped from tissues or blood vessels. Contain small amount of proteins or solids
What is the built-in prisim used for in the refractometer
Deviation and dispersion of light
What is special about the scale of the refractometer
It needs to be calibrated and tested. Measures specific gravity of urine and protein concentration in grams per 100 mL
What are the four components of the refractometer
Eyepiece, cover plate, prism, calibration screw
Describe the AO TS refractometer
Plasma protein and urine specific gravity. Measures refractive index, protein concentration of plasma, urine specific gravity.
What does a uricon refractometer measure
Urine specific gravity, refractive index scale
What does the Leica vet 360 refractometer measure
Urine specific gravity for dogs and cats as well as serum or plasma protein in grams per 100 mL
How do you use the refractometer
Please drop of sample on prison plus gently close cover plate. Look at scale through eyepiece. Read result.
How do you clean the refractometer
Wipe clean with tissue paper and distilled water
Why do you avoid any gritty material on the prism
So that it doesn’t scratch the lens
How often should you calibrate the refractometer
Weekly
With what fluid should you calibrate the refractometer
Distilled water. Scale should read zero
How do you calibrate the refractometer
With the adjusting screw.
Can you immerse the refractometer and water
No
What do we do blood testing for
Complete blood, biochemistry, coagulation profile, immunology testing, blood bank testing, DNA testing
When you do a biochem what are the components
Protein, glucose, lipids, electrolytes, enzymes, hormones
What is plasma and what is it used for
Plasma is obtained after centrifugation of blood with a tube containing an anticoagulant such as EDTA, sodium site trait, heparin. Can be used for biochemistry analysis and total protein
What are EDTA tubes used for
Plasma collection after centrifugation. Hematocrit determination by microhematocrit Capillary tube and a blood smear
What is serum and what is it used for
Obtained after centrifugation of blood that has been allowed to coagulate. The clot composition is red blood cells white blood cells platelets fibrin and coagulation factors. Serum is used for blood biochemistry, red top tubes, tiger top tubes
