Idexx In House Analyzers Flashcards

0
Q

What species can lasercyte hematology analyzer analyze samples from

A

Cat dog equine

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1
Q

What can the laser cyte hematology analyzer do

A

Check red blood cells, Reticulocytes, white blood cells, platelet count

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2
Q

Which white blood cells are granulocytes

A

All the Phils

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3
Q

Which white blood cells are agranulocyte’s

A

All the cytes

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4
Q

How does the lasercyte hematology analyzer work

A

Each cell goes through a laser beam. Light is scattered by the cell and measured by four detectors based on size, complexity, granularity, light absorption. The time of flight through the laser beam is also recorded

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5
Q

What are the main components of the lasersight hematology analyzer

A

Reagent compartment containing sheath reagent bottle and waste bottle. Air filter, status light, loading tray

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6
Q

In the CBC5R kit which tubes are contained

A

Purple top tubes, gray top tube, red top tubes, yellow top tubes

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7
Q

What is inside the purple top tube

A

EDTA

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8
Q

What’s inside the grey top tube

A

Quality beads, and new methylene blue stain to stain reticulocytes.

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9
Q

What is inside the red top tube

A

Discard tube

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10
Q

What is inside the yellow top tube

A

Automated enzymatic cleaner

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11
Q

What does the sheath reagent do

A

Lyses red blood cells to isolate the white blood cells for analysis

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12
Q

Go through the steps of analyzing a sample with the lasercyte

A

Collect a sample, Fill purple top that collect tubes with 0.5 to 1.5 mL. Invert eight times before collecting and running. Put sample and gray top tube in the analyzer. Enter patient info and run it.

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13
Q

What are the different ways you can verify results from the laser sight analyzer

A

Dot Plot, blood smear

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14
Q

How do you calibrate the laser sight analyzer

A

It’s done at the factory

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15
Q

How is quality control run on the lasersight analyzer

A

Quality beads in reagent tubes and two different hemoglobin readings are done

16
Q

How does maintenance on the lasersight analyzer work

A

It’s automated. After every sample, every four hours, and every 24 hours it flushes and rinses. After every 20 samples the extensive enzymatic clean happens. Weekly the air filter must be cleaned, the system is rebooted and backed up, sheath reagent amount is checked, waste bottle is emptied

17
Q

What does the catalyst DX analyzer do

A

Chemistry analysis, electrolytes, UPC fructosamine phenobarbital.

18
Q

How does the catalyst DX analyzer work

A

By chemical reaction

19
Q

What are the main components of the catalyst DX analyzer

A

Touchscreen interface, sample drawer, waste drawer, maintenance access doors, tip and diluent drawer

20
Q

What are the pre-loaded clips you can put into the catalyst DX on a laser

A

Chem: 17 for canine, chem: 15 feline, Chem: 10 Preanesthetic, Lyte 4: electrolytes

21
Q

What are some other main components of the catalyst DX analyzer

A

lithium Heprin whole blood separator, pipettes, sample cups

22
Q

How do you prepare a whole blood sample for the catalyst DX analyzer

A

.7 mL in lithium heparin separator. Swirl five times

23
Q

How do you prepare a plasma sample for the catalyst DX analyzer

A

Whole blood in lithium heparin tube, mix, centrifuge. 300 µL in sample cup with no bubbles

24
Q

How do you prepare a serum sample for the catalyst DX analyzer

A

Dry or SST tube, allowed to Claude, centrifuge, 300 µL in sample cup with no bubbles

25
Q

How do you prepare a urine sample for the catalyst DX analyzer

A

Centrifuge, 300 µL of supernatant in sample cup

26
Q

How do you analyze the sample in the catalyst DX analyzer

A

Select patient data, load sample, load clip or slides with the Lyte 4 first, run, view results on catalyst or vet lab machine.

27
Q

How do you maintain the catalyst DX analyzer

A

Empty the waste drawer, clean the internal components

28
Q

How do you clean the internal components of the catalyst DX analyzer

A

Do it monthly and before quality control, lint free wipes with 70% alcohol

29
Q

When do you do the quality control

A

Monthly,
after the analyzer has been moved
to verify system performance

30
Q

How do you do a quality control sample

A

Client equals QC, patient equals lot number of QC clip, sample equals 300 µL vettrol solution, load QC clip and lyte 4 clip, compare results to pass fail values on QC log

31
Q

What does the vet lab station do

A

Integrates results into a single report, organizes results, reduces manual data entry, integrates with clinics computer system