Red Blood Cell Parameters

Question 1

Hb

Answer 1

Total haemoglobin in g/l

Question 2

HCT

Answer 2

Haematocrit- packed red cell volume l/l

Question 3

MCV

Answer 3

Average volume of each rbc fl

Question 4

MCH

Answer 4

Average mass of haemoglobin in each rbc pg

Question 5

MCHC

Answer 5

average Hb concentration in each rbc g/l

Question 6

MCV calculation

Answer 6

HCT/RBC

Question 7

MCH CALCULATION

Answer 7

HB/RBC

Question 8

MCHC calculation

Answer 8

Hb/HCT

Question 9

Reduced MCV

Answer 9

Due to decreased hb synthesis

Question 10

Increased mcv

Answer 10

Lack of B12 or folate
Liver disease
Haemolysis
Pregnancy

Question 11

Causes of Haemolysis

Answer 11

Sickle cell anaemia
Heridetary spherocytosis
Incompatible blood transfusion
G6PDD

Question 12

Haemocytometer use

Answer 12

Cell count in a grid

Question 13

Purpose of EDTA

Answer 13

To prevent blood clottting

Question 14

Why does sickle cell anaemia haemoglobin travel further during electrophoresis

Answer 14

As a less negatively charged haemoglobin is made (glutamate changed to valine which has no charge)

Question 15

How to measure haemotocrit

Answer 15

Blood sample is centrifuged in a capillary tube.