Creatine Kinase Flashcards
Ck buffers system
Creatine phosphate is converted to Creatine and ATP catalyzed by Creatine kinase
Where is ck found
In brain and muscle cells
What are the isoenzymes of ck
MM found in muscle cells
MB found in myocardium
BB found in brain cells
MM form is the most positve
When is ck found in blood
After damage or death of brain cells
What causes the plasma membrane of myocardial cells to become leaky?
The semi-permeable membrane excludes certail molecules, such as Na+, so it needs a pump. This requires ATP. If there is less oxygen, pumps do not function, ion balance is lost and cells die, causing cell contents to be released
How might you determine CK activity
creatine phosphate+ADP=Creatine and atp
D-glucose+ATP=ADP+G6P .hexokinase.
G6P+NADP+==6-PG+NADPH+H+
Why might the three isoenzymes be separated by electrophoresis?
As they have different isoelectric points so different PH
Does an increase in serum CK activity relate to the size of myocardial damage?
CK BM isoform in the serum are directly proportional to the amount of cell death in the heart
What is the time course of serum CK after a myocardial infarction?
Peak: 1 day after MI
What other markers can be used for diagnosis of myocardial damage?
Troponin peaks 48H after MI
Serum glutamate oxaloacetate transaminase peaks after 2 days
Lactate dehydrogenase peaks after 5 days
What is myocardial infarct
Death if heart muscle cells occurs due to blockage if arteries called atherosclerosis
