REAGENT PREPARATION AND LABORATORY SUPPLIES

Question 1

Specifications established by the American Chemical Society (ACS); Labels on reagents state the actual impurities for each chemical lot OR list the maximum allowable impurities; Suitable for use in most analytic laboratory procedures

Answer 1

Analytic Reagent (AR)

Question 2

Additional purification steps; for use in specific procedures such as chromatography, atomic absorption, immunoassays, molecular diagnostics, standardization or other techniques that require extremely pure chemicals

Answer 2

Ultrapure

Question 3

Impurity limitations are not stated; preparation of these chemicals is not uniform; melting point analysis is often used to ascertain the acceptability purity range; not recommended for use unless further purification or a reagent blank is included

Answer 3

Chemically pure (CP) or pure grade

Question 4

Used to manufacture drugs; limitations are based on the criterion of not being injurious to individuals; purity standards are not based on laboratory needs

Answer 4

United States Pharmacopeia (USP)/ National Formulary (NF)

Question 5

used primarily in manufacturing and should never be used in the clinical laboratory

Answer 5

Technical/Commercial Grade

Question 6

Glass or plastic utensils used to transfer liquids

Answer 6

Pipets

Question 7

Holds or contains a particular volume but does not dispense that exact volume

Answer 7

To contain (TC) pipet

Question 8

will dispense the volume indicated

Answer 8

To deliver (TD) pipet

Question 9

continuous etched ring or two small, close continuous rings located near the top of the pipet

Answer 9

Blowout

Question 10

Allows the contents of the pipet to drain by gravity

Answer 10

Self-draining pipet

Question 11

What drainage characteristics does serologic pipets have?

Answer 11

Blowout

Question 12

What drainage characteristics does Mohr pipet have?

Answer 12

self-draining

Question 13

Dispense or transfer aqueous solutions; self-draining

Answer 13

Volumetric

Question 14

For biologic fluids having a viscosity greater than that of water, blow out

Answer 14

Ostwald-Folin pipet

Question 15

Most routinely used pipet

Answer 15

Automatic macropipets or micropipets

Question 16

Types of Automatic pipets:

Answer 16

1. Air-displacement pipets
2. Positive-displacement pipets

Question 17

Piston-operated devices. A disposable, one time use polypropylene tip is attached to the pipet barrel. The pipet is placed into the liquid to be aspirated and is drawn into a dispensed from this tip.

Answer 17

Air-displacement pipets

Question 18

Use a capillary tip that may be siliconized glass, glass, or plastic. This type of pipet is useful if a reagent reacts to plastics.

Answer 18

Positive-displacement pipets

Question 19

Allows the tubes to attain a horizontal position when spinning and a vertical position when the head is not moving; can attain speeds of up to approximately 3000 rpm

Answer 19

Horizontal or swinging bucket

Question 20

have angled compartments for the tubes and allow small particles to sediment more rapidly

Answer 20

Fixed-angle or angle-head

Question 21

The angle of the angle-head centrifuge is fixed at around:

Answer 21

52 degrees

Question 22

high-speed centrifuges used to separate layers of different specific gravities; they are commonly used to separate lipoproteins (used in the reference methods for lipoprotein quantitation)

Answer 22

Ultracentrifuges

Question 23

Centrifuge care:
- Calibration is done very:
- Disinfect on a:
- Check speed using:

Answer 23

Centrifuge care:
- Calibration is done very: 3 MONTHS (QUARTERLY)
- Disinfect on a: WEEKLY BASIS
- Check speed using: TIMER IS ALSO USED