Rbc Indices Flashcards
Disadvantages of a automated analyzer
Increased hazards, physical, electric, chemical
Two techniques.
Electrical impedance counting, light scatter cell counting
How does electrical impedance work?
Cells are distributed in the solution, cells pass through an opening and interrupt the signal causing a pulse which counts the cell
How does light scatter method work?
A laser beam hits each cell causing the light to scatter and counts the cell based off scatter type
What do three part differentials look at?
Shrinks cytoplasm, monocytes and granulocytes
What does a five part differential look at?
Neutrophils, lymphocytes, monocytes, eosinophils, basophils
Advantages of chemistry analyzers.
Easy to use, no handling reagents, smaller samples required.
Disadvantages of chemistry analyzers
Expense, maintenance required, reagents take up spaces in fridge and need to be warmed
Main use for rbc indices
Evaluating anemia, dogs most applicable, looks at cell size and hemoglobin concentration
Three tests needed for rbc indices.
Hemoglobin, hematocrit, rbc count
Mcv
Mean cell volume- how big (size)
Mch
Mean cell hemoglobin - average weight of hgb
Mchc
Mean cell hemoglobin concentration- concentrationn relation to cell size
Normal Mcv vaules
60-77 dogs , 39-55 cats (fl)
Normal Mchc values?
Dog 32-36 g/Dl, cat 30-36 g/Dl
