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Hematology > Hct, Pcv, Blood Smear Review > Flashcards

Hct, Pcv, Blood Smear Review Flashcards

1
Q

Normal pcv for dogs?

A

37-55%

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2
Q

Normal pcv of cats?

A

35-45%

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3
Q

What is the white called between the rbcs and plasma of hematocrit tubes?

A

Buffy coat

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4
Q

What do you use the refractometer to find out?

A

Total protein

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5
Q

What is the of unit of total protein?

A

g/100ml

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6
Q

Normal plasma total protein of dogs and cats?

A

6.0-7.5 g/dL

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7
Q

What do you do before using a refractometer?

A

Calibrate with distiller water

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8
Q

What is normal plasma color?

A

Straw yellow

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9
Q

What does white plasma mean?

A

Lipemic-lipids in blood.

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10
Q

What does yellow plasma mean?

A

Icteric-liver is having problems

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11
Q

What does red/pink plasma mean?

A

Hemolysis- ruptured rbcs, heat, freezing, small gauge needle

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12
Q

What do we look for in blood smears?

A

Confirm cell numbers, differential count, cell morphology, blood borne parasites

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13
Q

Best tube to do a blood smear with?

A

EDTA, purple top

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14
Q

Why do we use purple tops for blood smears? (Cbc)

A

Preserves blood morphology best

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15
Q

Steps of blood smears?

A

Clean, dry slide, mix sample well, put a small drop on the slide, hold the spreader at 40 degrees, back the spreader into the drop, push the spreader forward, smooth feathered edge

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16
Q

Why do we not apply pressure during blood smears?

A

May rupture/squish cells

17
Q

What should the smear look like?

A

A thumb with a feathered edge

18
Q

What happens if you increase the angle of the blood smear pusher?

A

It will be a thicker smear

19
Q

What happens if you decrease the angle of the blood smear pusher?

A

Make a thinner smear

20
Q

What are the here distinct areas of a blood smear?

A

Thick blood cell area, red cell area, feathered edge

21
Q

What do we use the thick blood cell area for?

A

Nothing really

22
Q

What do we use the red cell area of a smear for?

A

To view morphology, differential- they’re spaced out well

23
Q

What do we use the feathered edge of a smear for?

A

To check for clumped platelets and heartworm microfilariae

24
Q

If the smear is too short and thick?…

A

Make the drop smaller and decrease spreader angle

25
Q

If the blood smear is too thin…?

A

Increase blood drop, increase angle of spreader and apply less pressure

26
Q

Steps to dying blood smear?

A

Let air dry!, stain (fixative-blue), eosin stain (red), methylene blue (dark blue), rinse with water, air dry

27
Q

How long do we have to make a slide and then to stain?

A

3 hours after collecting, one hour after slide is made

28
Q

What is the best way to scan a smear?

A

Zig zag pattern

29
Q

What you see when you look into the microscope?

A

Field of view

30
Q

What are we looking for with red blood cell morphology?

A

Shape and size normal?

31
Q

What are we looking for with white blood cell morphology?

A

Neutrophils mature? Lymphocytes normal? Wbc inclusions?

32
Q

What are we looking for for platelet morphology?

A

Normal numbers? Normal size?

33
Q

The percentage of whole blood that Is composed of red blood cells.

A

Hematocrit, packed blood cell volume

34
Q

What do total proteins tell us?

A

Amount of proteins in the blood- especially proteins regarding liver

35
Q

If we have an anemic patient, what happens to our pcv?

A

Lower packed cell volume

36
Q

If our patient is dehydrated,what happens to our pcv?

A

Increased

37
Q

If our patient is dehydrated, what will happen to our total protein?

A

Increase, it’s not spread out

38
Q

Here parts of a blood slide?

A

Thick body area, red cell rainbow counting area, feathered edge

Hematology (15 decks)

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