RBC Count Flashcards
Number of Erythrocytes contained in 1 liter of blood
Erythrocyte Number Concentration
Erythrocyte Number Concentration is expressed as?
Number of Cells per Cubic Millimeter
RBC Count can be used to help diagnose? (2)
- Anemia
- Polycythemia
Manual Methods to Count RBC
- Microscopic
- Unopette System
- Tube Dilution
RBC Pipette can aspirate up to?
0.5 - 1.0
Dilution Factor of RBC Pipette
200
Why is EDTA used for RBC Counting?
Retains Cell Morphology
How many squares are counted in RBC Count?
5 Tertiary Squares
Characteristics of Good RBC Diluting Fluid (7)
- Isotonic Solution
- High SG
- Easy to Prep
- Cheap
- Good Preservative
- Buffer Action
- Not initiate growth of molds
RBC Diluting Fluids (7)
- Dacie’s
- Hayem’s
- Gower’s
- Toisson’s
- NSS
- Bethell’s
- 3.8% Sodium Citrate
Best RBC Diluting fluid and can be stored for long periods of time
Dacie’s / Formol Citrate
Best RBC Diluting fluid and can be stored for long periods of time
Dacie’s / Formol Citrate
This Diluting Fluid allows growth of yeast, Clumping of cells and has no corrosive effect
Hayem’s
This diluting fluid prevents rouleaux formation and precipitates protein
Gower’s
Diluting fluid that support growth of fungi with High SG and can also stain WBCs
Toisson’s
Used in emergency cases and can cause excessive rouleaux formation
NSS
Angle of Pipette while charging?
30-35 Degrees
Stand the Counting chamber for how many minutes?
5-10 mins
Reference Range for RBC Count?
Male: 4.5-6 M/cumm
Female: 4.0-5.5 M/cumm
Late Pregnancy: 3-5 M/cumm
At Birth: 7.0 M/cumm
3 Errors due to Materials and Equipment
- Debris in coverslip/chamber
- Old Diluting Fluid
- RBC Pipette not clean
6 Errors due to Nature/Operators error
- Incorrect Dilution
- Did not Discard
- Overcharging
- Undercharging
- Air bubbles
- Incorrect Counting
If RBC is markedly increased?
Decrease Volume of Aspirated Blood
If RBC is markedly decreased?
Increase Volume of Aspirated Blood
What may result to increased RBC Count? (2)
- Dehydration
- Strenuous exercise Activities
5 Pathologic Variations of RBC Count?
- Polycythemia
- Anemia
- Hemorrhage
- Drugs
- Secondary to other diseases
4 Types of Counting Chamber
- Improved Neubauer
- Neubauer
- Fuchs-Rosenthal
- Speirs-Levy
Depth of Improved Neubauer
0.1 mm