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HEMA LAB > RBC Count > Flashcards

RBC Count Flashcards

1
Q

Number of Erythrocytes contained in 1 liter of blood

A

Erythrocyte Number Concentration

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2
Q

Erythrocyte Number Concentration is expressed as?

A

Number of Cells per Cubic Millimeter

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3
Q

RBC Count can be used to help diagnose? (2)

A
  1. Anemia
  2. Polycythemia
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4
Q

Manual Methods to Count RBC

A
  1. Microscopic
  2. Unopette System
  3. Tube Dilution
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5
Q

RBC Pipette can aspirate up to?

A

0.5 - 1.0

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6
Q

Dilution Factor of RBC Pipette

A

200

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7
Q

Why is EDTA used for RBC Counting?

A

Retains Cell Morphology

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8
Q

How many squares are counted in RBC Count?

A

5 Tertiary Squares

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9
Q

Characteristics of Good RBC Diluting Fluid (7)

A
  1. Isotonic Solution
  2. High SG
  3. Easy to Prep
  4. Cheap
  5. Good Preservative
  6. Buffer Action
  7. Not initiate growth of molds
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10
Q

RBC Diluting Fluids (7)

A
  1. Dacie’s
  2. Hayem’s
  3. Gower’s
  4. Toisson’s
  5. NSS
  6. Bethell’s
  7. 3.8% Sodium Citrate
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11
Q

Best RBC Diluting fluid and can be stored for long periods of time

A

Dacie’s / Formol Citrate

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12
Q

Best RBC Diluting fluid and can be stored for long periods of time

A

Dacie’s / Formol Citrate

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13
Q

This Diluting Fluid allows growth of yeast, Clumping of cells and has no corrosive effect

A

Hayem’s

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14
Q

This diluting fluid prevents rouleaux formation and precipitates protein

A

Gower’s

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15
Q

Diluting fluid that support growth of fungi with High SG and can also stain WBCs

A

Toisson’s

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16
Q

Used in emergency cases and can cause excessive rouleaux formation

A

NSS

17
Q

Angle of Pipette while charging?

A

30-35 Degrees

18
Q

Stand the Counting chamber for how many minutes?

A

5-10 mins

19
Q

Reference Range for RBC Count?

A

Male: 4.5-6 M/cumm
Female: 4.0-5.5 M/cumm
Late Pregnancy: 3-5 M/cumm
At Birth: 7.0 M/cumm

20
Q

3 Errors due to Materials and Equipment

A
  1. Debris in coverslip/chamber
  2. Old Diluting Fluid
  3. RBC Pipette not clean
21
Q

6 Errors due to Nature/Operators error

A
  1. Incorrect Dilution
  2. Did not Discard
  3. Overcharging
  4. Undercharging
  5. Air bubbles
  6. Incorrect Counting
22
Q

If RBC is markedly increased?

A

Decrease Volume of Aspirated Blood

23
Q

If RBC is markedly decreased?

A

Increase Volume of Aspirated Blood

24
Q

What may result to increased RBC Count? (2)

A
  1. Dehydration
  2. Strenuous exercise Activities
25
Q

5 Pathologic Variations of RBC Count?

A
  1. Polycythemia
  2. Anemia
  3. Hemorrhage
  4. Drugs
  5. Secondary to other diseases
26
Q

4 Types of Counting Chamber

A
  1. Improved Neubauer
  2. Neubauer
  3. Fuchs-Rosenthal
  4. Speirs-Levy
27
Q

Depth of Improved Neubauer

A

0.1 mm

HEMA LAB (9 decks)

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