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HEMA LAB > Hematocrit Determination > Flashcards

Hematocrit Determination Flashcards

1
Q

Volume of packed red blood cells that occupies a given volume of whole blood after centrifugation

A

Hematocrit

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2
Q

Hematocrit is also known as? (2)

A
  1. Packed Cell Volume
  2. Erythrocyte Volume Fraction
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3
Q

4 Ways to Report Hematocrit

A
  1. Percentage
  2. Cell Volume Percent
  3. Volume Percent
  4. Liters per Liter
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4
Q

4 Components of Plasma

A
  1. Water
  2. Proteins
  3. Nutrients
  4. Hormones
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5
Q

2 Components of Buffy Coat

A
  1. WBCs
  2. Platelets
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6
Q

Normal Range of Hematocrit for Females?

A

35-49%

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7
Q

Normal Range of Hematocrit for Males?

A

40-54%

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8
Q

2 Methods of Hematocrit Determination

A
  1. Microhematocrit
  2. Macrohematocrit
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9
Q

Method used to Microhematocrit?

A

Adam’s Microhematocrit Method

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10
Q

Anticoagulant used for Microhematocrit Method (2)

A
  1. Edta
  2. Heparin
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11
Q

Fill the capillary tubes with approximately how much blood?

A

3/4 full / 0.05 ml

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12
Q

Length of Capillary Tubes

A

7-7.5 cm

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13
Q

Bore size of Capillary Tubes

A

1.2 mm

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14
Q

Capillary tube for anticoagulated blood

A

Blue / Non-Heparinized

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15
Q

Capillary tube used for skin puncture

A

Red / Heparinized

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16
Q

The seal of the capillary tubes should be atleast ___ long

A

4 mm

17
Q

While centrifuging the capillary tubes the clay ends should face?

A

Away from center

18
Q

centrifuge Microhematocrit tubes at ______ to _____ for ____ minutes

A

10k - 15k for 4-5 mins

19
Q

The value of duplicate hematocrits should agree within?

A

1% / 0.1 L/L

20
Q

1% hematocrit is equal to?

A

0.34 g/dl Hgb / 107k RBC/cumm

21
Q

What are the possible errors that causes Decreased Results? (6)

A
  1. Improper Sealing of Capillary Tubes
  2. Short draw in evacuated tube
  3. Specimen was not mixed properly
  4. Microhematocrit Reader is not used properly
  5. Interstitial Fluid from skin puncture
  6. Improper Flushing of intravenous catheter
22
Q

What are the possible errors that cause increase in hematocrit reading? (3)

A
  1. Buffy coat is included
  2. Dehydration
  3. Reader is not used Properly
23
Q

5 Disorders that may cause plasma to be trapped in the RBC Layer

A
  1. Sickle Cell Anemia
  2. Thalassemia
  3. Macrocytic Anemia
  4. Spherocytosis
  5. Hypochromic Anemia
24
Q

Trapping of plasma causes Microhematocrit to be ____ higher than the value obtained using automated instruments

A

1-3%

25
Q

Formula for Hematocrit Determination using Automated Methods

A

Hct = RBC x MCV

26
Q

2 Automated Methods for Hematocrit

A
  1. Coulter Counter
  2. Autoanalyzer
27
Q

What causes a decrease in plasma volume but increase in hematocrit reading?

A

Dehydration

28
Q

Method used for Macrohematocrit?

A

Wintrobe Method

29
Q

Length of Wintrobe Tube

A

11.5 cm

30
Q

Bore of Wintrobe Tube

A

3 mm

31
Q

Calibration used for Hematocrit Determination in Wintrobe Tube?

A

White / 10-0

32
Q

0-10 or Red Calibration of Wintrobe Tube is used for?

A

Erythrocyte Sedimentation Rate

33
Q

Anticoagulant of choice for Wintrobe Method?

A

Double Oxalate

34
Q

Centrifuge the Wintrobe Tube at ___ rpm for how many minutes?

A

3k for 30 mins

35
Q

Hematocrit Determination Formula for Wintrobe Method?

A

Hct = (Height of Packed RBC / Height of Whole Blood) x 100

36
Q

The Rule of 3 only applies to specimens that are?

A

Normocytic Normochromic RBCs

HEMA LAB (9 decks)

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