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HEMA LAB > CyanMethemoglobin Method > Flashcards

CyanMethemoglobin Method Flashcards

1
Q

Hemoglobin is measured indirectly by converting it into? (4)

A
  1. Cyanmethemoglobin
  2. Acid Hematin
  3. Alkali Hematin
  4. Carboxyhemoglobin
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2
Q

Cyanmethemoglobin Method is also known as?

A

Hemoglobin Cyanide (HiCN)

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3
Q

Advantages of Cyanmethemoglobin (4)

A
  1. Stable in Dilution
  2. Commercially Available
  3. All forms except Sulfhemoglobin
  4. Allow different types of spectrophotometer
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4
Q

Drabkin Solution Composition (4)

A
  1. Potassium Ferricyanide
  2. Potassium Cyanide
  3. Sodium Bicarbonate
  4. Surfactant
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5
Q

Principle of Cyanmethemoglobin Method

A

Hemoglobin is Oxidized from Ferrous to ferric state by Potassium Ferricyanide to form Methemoglobin. Then Potassium Cyanide converts methemoglobin to Cyanmethemoglobin

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6
Q

pH for Sodium Bicarbonate substitute

A

7.0-7.4

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7
Q

What does substituting Sodium Bicarbonate do to the Hemoglobin?

A

shortens time for conversion from 10 mins to 3 mins

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8
Q

What is the wavelength needed for spectrophotometer in Cyanmethemoglobin Method?

A

540 nm

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9
Q

The absorbance is directly _____ to the ______

A

Proportional / Hemoglobin Concentration

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10
Q

How many minutes do you stand the solution for?

A

10 mins

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11
Q

3 Sources of Error for Cyanmethemoglobin Method

A
  1. Reagent is Light Sensitive
  2. Reagent is Highly Toxic
  3. Time Consuming and Erroneous delivery Results due to Heavy Smoker
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12
Q

Turbidity will cause?

A

False-Increase Result

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13
Q

High WBC Count?

A

> 20x10^9/L

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14
Q

High Platelet Count?

A

> 700x10^9/L

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15
Q

Remedy for High Platelet Count?

A

Reagent and Specimen should be Centrifuged and Supernatant is measured

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16
Q

Remedy for Lipemic Patient

A

Add 0.01 ml of plasma to 5ml of Reagent to use as blank

17
Q

Remedy for Hemoglobin S and Hemoglobin C

A

1:2 Dilution with distilled water (1 Part Specimen 1 Part water) then multiply result by 2

18
Q

Abnormal Globulins can be found in patients with? (2)

A
  1. Plasma Cell Myeloma
  2. Waldenstrom Microglobulinemia
19
Q

Remedy for Abnormal Globulin?

A

Add 0.1g of Potassium Carbonate to Reagent

20
Q

Reference Values for Cyanmethemoglobin Method

A

Male: 14-18 g/dl
Female: 12-15 g/dl

HEMA LAB (9 decks)

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