OFT Flashcards
RBCs placed in Hypotonic Solution causes them to?
Swell
What leades to accumulation of water in RBCs?
Increased Permeability
OFT is a screening test for?
Hereditary Spherocytosis
Test that demonstrates increased RBC Fragility
OFT
RBCs that have Decreased Surface Area to Volume Ratio?
Spherocytes
This is Caused by lack of vertical interaction of RBC Membrane which is a result of ______
Hereditary Spherocytosis/ Spectrin Deficiency
In WAHA, RBCs are sensitized by antibodies known as _____
IgGs
OFT lacks ____ and ____ because it cannot differentiate HS or WAHA
Specificity and Sensitivity
Principle of OFT
Whole Blood is added to hypotonic solutions that being buffered sodium chloride solution and allow to incubate at RT
4 Precautions of OFT
- Sample with Minimum Stasis and Trauma
- Test should be set ASAP
- Uniform Size of drops
- Blood should fall directly into saline solution
Tube used for OFT?
Heparin (Green)
Hypotonic Solution Used?
0.5% NaCl
Rejection Criteria for OFT?
Hemolyzed Specimen
0.5% NaCl Solution consist of? (2)
0.5g NaCl in 100ml D Water
Name of 12 tubes used for OFT?
Wasserman
How long do you stand the tubes for?
2 Hours
Method used for OFT?
Griffin-Sandford Method
Computation for Griffin-Sandford Method
Tube # x 0.02
Color of Initial Hemolysis
Pink
Color of Complete Hemolysis
Red
Cell button is present
Initial Hemolysis
Initial Hemolysis normally starts at what tubes?
22 & 21
Complete Hemolysis normally starts at what tubes?
16 & 17
Reference Value of Griffin-Sandford Method
Initial : 0.42-0.44%
Complete: 0.32-0.34%
Shift to the Left indicates?
Increased OFT
Examples of Increased OFT (2)
- Spherocytes
- Older RBCs
Shift to the Right Indicates?
Decreased OFT
Examples of Decreased OFT? (3)
- Thalassemia
- Hemoglobinopathies
- Reticulocytes
8 Factors that affect OFT
- Shape
- Functional State
- Surface to Volume Area
- Chemical Purity
- Accuracy of NaCl
- Tonicity of Solution
- pH
- Temperature
in OFT, pH level must be?
7.4
A change of pH by 0.1 is equal to the ______ by _____
Change of Tonicity by 0.01%
5°c change is equal to a change of tonicity by?
0.01%
Increased OFT indicates? (4)
- Decreased Resistance
- HA
- HS
- Spherocytes
Decreased OFT Indicates? (8)
- Increased Resistance
- Splenectomy
- Liver Disease
- Sickle Cell Anemia
- IDA
- Thalassemia
- Polycythemia Vera
- Target Cells