RAT #9 - Transcriptional Regulation - DNA Sequence Dependent Flashcards

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1
Q

The proteins which bind to DNA sequences to form enhanceosomes are called?

a. repressors
b. motivators
c. stimulators
d. activators

A

d. activators

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2
Q

Enhancers are found?

a. Downstream of the Start site
b. Within the Coding Region
c. Upstream from the Start site
d. in any of the above regions

A

d. in any of the above regions

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3
Q

Which of the following Transcriptional Regulatory elements provides the “best”, “most efficient” Transcription?

a. promoter
b. cis-acting element
c. start site
d. enhancer

Question 4
A promoter is:

a. a manager for a sports team
b.
a specific sequence of DNA to which RNA polymerase binds with help of general transcription factors
c. a specific DNA sequence to which micrococcal nucleases bind to d. do not exist in eukaryotic cells

A

d. enhancer

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4
Q

Question 4
A Promoter is:

a. a Manager for a Sports team
b. a Specific Sequence of DNA to which RNA Polymerase binds w/ help of General Transcription Factors
c. a Specific DNA sequence to which Micrococcal Nucleases bind to
d. Do NOT exist in Eukaryotic cells

A

b. a Specific Sequence of DNA to which RNA Polymerase binds w/ help of General Transcription Factors

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5
Q

Which of the following DNA sequences is ABSOLUTELY necessary for transcription to occur (in other words, without it, transcription would never occur)?

a. Enhancer
b. Promoter
c. Silencer
d. Proximal Control element

A

b. Promoter

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6
Q

DNA sequences that prevent enhancers from acting on promoters are called?

a. boundaries
b. isolators
c. silencers
d. insulators

A

c. Silencers

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7
Q

Methylated genes, relative to unmethylated genes, in Eukaryotic cells:

a. is Equivalent in Transcriptional activity
b. Do NOT exist in Eukaryotes
c. are MORE Transcriptionally Active
d. are LESS Transcriptionally Active

A

d. are LESS Transcriptionally Active

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8
Q

What is now viewed as the role of methylation of DNA in the process of transcription?

a. Methylation is irrelevant to Gene Transcription
b. to Prevent Mutations
c. to Interfere with DNA Transcription
d. to Complex with Enhancers to Promote Transcription

A

INCORRECT ⎃
b. to Prevent Mutations

d. to Complex with Enhancers to Promote Transcription (?)

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9
Q

What are Transcription Factors?

a. Exist only in Prokaryotes.
b. DNA sequences that function as Promoters.
c. Proteins that are required to Translate mRNA into Proteins.
d. Proteins which Bind to DNA + Regulate Transcription.

A

d. Proteins which Bind to DNA + Regulate Transcription

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10
Q

Which of the following are NOT Regulatory Transcription Factors?

a. TFIID
b. Repressors
c. Factors that Bind to cis-acting elements
d. Activators

A

a. TFIID

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