RAT #20 - Overview of Protein Transport + Trafficking

Question 1

The KDEL Signal works by Keeping or Retaining ER Lumenal and Membrane Proteins in the ER.

True
False

Answer 1

FALSE, (explain)

Question 2

The Lysosomal Targeting Signal is a Post-Translational Modification

True
False

Answer 2

TRUE

Question 3

The nuclear localization signal is a post-translational modification.

True
False

Answer 3

FALSE, (explain)

Question 4

Secretory proteins are synthesized by

a. Ribosomes on the Endoplasmic Reticulum
b. Free Ribosomes
c. Ribosomes on the Nuclear Membrane
d. All of the above

Answer 4

INCORRECT

d.

Question 5

Signal sequences are

a. Short peptide sequences attached to a protein that initiates its degradation by digestive enzymes
b. Short Peptide Sequences that serve as an Address for Transporting Newly Synthesized Proteins to the Correct Location
c. Glycoproteins that serve as an address for transporting newly synthesized proteins to the correct location
d. Short peptide sequences to transport a protein to the nucleus

Answer 5

b. Short Peptide Sequences that serve as an Address for Transporting Newly Synthesized Proteins to the Correct Location

Question 6

Proteins required in the Cytosol like the Enzymes of Glycolysis are Synthesized on

a. Ribosomes on the Nuclear Membrane
b. Ribosomes on the Endoplasmic Reticulum
c. Free Ribosomes in the Cytosol
d. Smooth Endoplasmic Reticulum

Answer 6

INCORRECT

B.

Question 7

The process of sorting and transporting newly synthesized proteins to their correct destination in a cell is called?

a. Protein Sorting
b. Protein Targeting
c. Protein Trafficking
d. All of these

Answer 7

d. All of these

Question 8

Proteins containing an NLS are part of the secretion pathway.

True
False

Answer 8

FALSE, (explain)

Question 9

Choose the CORRECT Order of Transport of Proteins in the Secretory Pathway.

a. Protein Synthesized in the Cytoplasm >SER lumen > RER Lumen > cis Golgi >Median Golgi > trans Golgi > Vesicles > Fusion of Vesicles w/ Plasma Membrane > Exoctyosis
b. Protein Synthesized in the Cytoplasm > RER Lumen > cis Golgi >Median Golgi > trans Golgi > Vesicles > Fusion of Vesicles w/ Plasma Membrane > Exoctyosis
c. Protein Synthesized in the Cytoplasm > Vesicles > SER > RER Lumen > cis Golgi > Median Golgi > trans Golgi > Vesicles > Fusion of Vesicles w/ Plasma Membrane > Exoctyosis
d. Protein Synthesized in the Cytoplasm > RER Lumen > trans Golgi > Median Golgi > cis Golgi > Vesicles > Fusion of Vesicles w/ Plasma Membrane > Exoctyosis

Answer 9

b. Protein Synthesized in the Cytoplasm > RER lumen > cis Golgi >Median Golgi > trans Golgi > Vesicles > Fusion of Vesicles w/ Plasma Membrane > Exoctyosis

Question 10

According to the Signal Hypothesis, the Signal Peptide for a Secretory Protein is?

a. Rich in Hydrophilic Amino Acids @ the Center of the Signal Peptide + is Located @ the C-terminus of the Protein
b. Rich in Hydrophilic Amino Acids @ the Center of the Signal Peptide + is Located @ the N-terminus of the Protein
c. Rich in Hydrophobic Amino Acids @ the Center of the Signal Peptide + is Located @ the C-terminus of the Protein
d. Rich in Hydrophobic Amino Acids @ the Center of the Signal Peptide + is Located @ the N-terminus of the Protein

Answer 10

d. Rich in Hydrophobic Amino Acids @ the Center of the Signal Peptide + is Located @ the N-terminus of the Protein