Raf, MEK and ERK

Question 1

Raf is activated by_____________.

Answer 1

Ras/GTP

Question 2

Raf has a __________ domain that keeps it in a _________ conformation by interacting with _______ and ______

Answer 2

14-3-3
Closed
Ser259
Ser621

Question 3

What recruits raf to the membrane?

Answer 3

Binding of ras/GTP to Arg89

Question 4

What sort or phosphorylation results in activation of MEK?

Answer 4

Serine and threonine

Question 5

Activated MEK binds to a __________ in the target protein

Answer 5

CD domain

Question 6

MEK phosphorylates _______ on _________ and _____________

Answer 6

ERK
Threonine
Tyrosine

Question 7

The signal from ______ to the nucleus can be via ______________, ____________________ eg____________ or _____________________

Answer 7

ERK
Direct translocation
Translocation of a substrate phosphorylated by ERK
Rsk
Phosphorylation of an inhibitor allowing the release of a transcription factor.

Question 8

Nuclear transcription factors are targets for ______.

There are parallel pathways for ____, ______ and ________.

Answer 8

ERK
JNK
ERK
p38