Quizzes 1-6

Question 1

The presence of blood group A is confirmed by detecting with anti-A the presence of which terminal sugar molecule?

Answer 1

N-acetyl-D-galactosamine

Question 2

The mating of parents (ew) of which two ABO phenotypes can potentially produce offspring with ALL of the common four blood groups?

Answer 2

A + B

Question 3

Which ABH substances would you expect to find in the saliva of a group B secretor?

Answer 3

H + B

Question 4

A blood donor has the genotype hh, AB. What is his apparent red cell phenotype during routine forward and reverse grouping?

Answer 4

O

Question 5

Which of the following genes codes for secretor status

Answer 5

Se

Question 6

The labels came off your reagent bottles. What color is the anti-B reagent?

Answer 6

yellow

Question 7

Most blood group systems are inherited as

Answer 7

codominant

Question 8

What is the ABO group of the individual with the following serologic reactions:
Anti-A = 0, Anti-B= 0,
A1 cells = 4+, B cells = 4+

Answer 8

O

Question 9

When a Ce haplotype is in trans to a D antigen encoding haplotype, which may occur

Answer 9

weak D phenotype with 1+ agglutination

Question 10

A new blood bank specimen is required 24-48 hours following a blood transfusion because

Answer 10

secondary immune response against donor antigens

Question 11

Which of the following is the most common subgroup of blood group A?

Answer 11

A1

Question 12

Which is true regarding the anti-A1 antibody in an A2 individual?

Answer 12

Group O blood should be transfused if the anti-A1 reacts at 37°C

Question 13

The plant lectin extracted from Dolichos biflorus will agglutinate

Answer 13

A1 cells but not A2 cells

Question 14

Which of the following blood groups possesses the most H antigen?

Answer 14

Group O

Question 15

All A subgroups always have which blood group circulating antibody

Answer 15

Anti-B

Question 16

Identify the strength of the agglutination reaction that provides the best hint as to the type of ABO discrepancy

Answer 16

weakest

Question 17

Which subgroup of blood group A shows mixed field agglutination with anti-A reagents?

Answer 17

A3

Question 18

What is the ABO group of the individual with the following serologic reactions: Anti-A = +4, Anti-B= +4, A 1 cells = 0, B cells = 0, anti-D = 4+

Answer 18

AB positive

Question 19

The observation of hemolyzed serum or plasma in a blood bank specimen is reason for

Answer 19

Rejecting the specimen

Question 20

The coin-like stacking of RBCs that may cause an ABO discrepancy is called

Answer 20

Rouleaux formation, Group III

Question 21

Rz phenotype is

Answer 21

DCE

Question 22

r” phenotype is

Answer 22

cE

Question 23

Weak D antigens

Answer 23

usually require an IAT phase for detection

Question 24

Which is true of partial D?

Answer 24

partial D individuals may not be detected until develop the anti-D antibody

Question 25

A serologic characteristic of the Del type of weak D antigen is

Answer 25

weak or undetectable agglutination reactions at IS (immediate spin)

Question 26

Rh system antibodies

Answer 26

show dosage

Question 27

Individuals of the Rh null phenotype produce which types of red cells?

Answer 27

stomatocytes

Question 28

Which of the following constitutes the f antigen?

Answer 28

c & e on the same chromosome

Question 29

What is the ABO group and Rh type of the individual with the following serologic reactions? Anti-A = 0 Anti-B = 0 A cells = 4+ B cells = 4+ anti-D = 1+

Answer 29

O weak D

Question 30

Identify the possible cause(s) for false positive reactions with Rh antibodies

Answer 30

Fibrin interference

Question 31

Which of the following antigens are destroyed by ficin (and papain)?

Answer 31

Duffy

Question 32

A common cause of delayed type hemolytic transfusion reactions are

Answer 32

anti-Jka and anti-Jkb

Question 33

McLeod phenotype

Answer 33

presence of reticulocytes and hyperbilirubunemia

Question 34

Which antigen is the second most antigenic after the D antigen

Answer 34

Kell

Question 35

What is the ABO group and Rh type of an individual with the following serologic reactions: Anti-A = +4, Anti-B= 0, A 1 cells = 0, B cells = 4+, anti-D = 0

Answer 35

A negative

Question 36

Kidd blood group system

Answer 36

antibodies usually bind complement

Question 37

What is the next step if a patient with anti-M reacts at 37°C is crossmatch compatible at 37°C and negative at the anti-human globulin (AHG) phase

Answer 37

provide donor RBCs without phenotyping for the M antigen

Question 38

Identify the high frequency (prevalence) antigen

Answer 38

Cellano

Question 39

Identify the blood group system that has the highest percentage of antigen negative RBC units

Answer 39

Kell

Question 40

Identify the characteristic associated with the Duffy antigen system

Answer 40

antibodies against Duffy antigens react at the AHG phase

Question 41

1 What is the predominant major cause of an ABO mistransfusion?

Answer 41

clerical error in identifying patient by the phlebotomist when drawing the T&S

Question 42

How should a patient be identified when drawing lab samples, processing lab samples, transfusing?

Answer 42

By double or preferably triple unique identifiers

Question 43

What is the maximum age a T&S specimen for Blood Bank can be when a patient has been transfused or pregnant in the last 3 months?

Answer 43

48 - 72 hours

Question 44

Which of the following tests are not required to be performed on all donor blood?

Answer 44

CMV

Question 45

What is the minimum time after transfusion must a T&S and a segment of donor blood be retained?

Answer 45

7 days

Question 46

What confirmatory testing must be performed on donor blood units by the blood bank transfusing the patient?

Answer 46

front type and Rh D if the unit is Rh negative

Question 47

When may an Immediate Spin Crossmatch be performed?

Answer 47

Correct When the antibody screen is negative and there is no history of alloantibodies

Question 48

The criteria for an incompatible cross-match or positive IAT is/are:

Answer 48

Agglutination and/or hemolysis

Question 49

AHG is used to identify:

Answer 49

RBCs sensitized with IgG alloantibodies after 37C incubation

Question 50

What does the IAT and cross-match have in common

Answer 50

Both test recipient serum or plasma that may have alloantibodies

Question 51

What is/are the criteria for a positive cross-match therefore rejecting the donor RBC unit

Answer 51

Hemolysis and/or agglutination

Question 52

Screening cells are all group O so as not to have interference by

Answer 52

anti-A or anti-B antibodies

Question 53

Using a three cell screen, you observe that the reactions are 3+, 0, 1+ at Coombs respectively. This suggests the presence of:

Answer 53

A dosage effect

Question 54

Identify the reagent that can act as agglutination potentiator, can cause non-specific agglutination, should not do a 37C reading and finish with AHG macroscopically

Answer 54

PEG

Question 55

A key characteristic of a positive reaction (agglutination) with the gel card system is:

Answer 55

IgG in the gel traps sensitized RBC so they remain at the top of the gel

Question 56

Antibody identification using a panel (of donor RBCs) requires as the first step:

Answer 56

Rule out all reagent RBCs that give negative reactions with homozygous antigen phenotype in all phases of testing

Question 57

You ran out of check cells. What is your next step:

Answer 57

Sensitize Rh positive RBCs with reagent anti-D

Question 58

Does the strength of agglutination with AHG signify clinical significance?

Answer 58

No